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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 8, Iss. 8 — Sep. 4, 2013

Quantitative Determination of Citric Acid in Seminal Plasma by Using Raman Spectroscopy

Zufang Huang, Xiwen Chen, Yongzeng Li, Jinhua Chen, Juqiang Lin, Jing Wang, Jinping Lei, and Rong Chen

Applied Spectroscopy, Vol. 67, Issue 7, pp. 757-760 (2013)


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Abstract

In this study, Raman spectroscopy was first used to study the linear relationship between Raman spectral intensities and citric acid concentrations in aqueous solution. By using the specific Raman band of 942 cm?1, concentrations of citric acid ranging from 2 to 20 mg/mL were observed linearly (R2 = 0.993), and the limit of detection was 1.0 mg/mL. Then, citric acid detection in clinical seminal plasma ultrafiltrate samples was performed, and the intensity of the Raman-specific peak demonstrates a good linear correlation (R2 = 0.946) with citric acid concentrations determined by the enzymatic method. Our results showed that Raman spectroscopy has the potential of being applied to detect concentrations of citric acid in seminal plasma in clinic.

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Vol. 8, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Zufang Huang, Xiwen Chen, Yongzeng Li, Jinhua Chen, Juqiang Lin, Jing Wang, Jinping Lei, and Rong Chen, "Quantitative Determination of Citric Acid in Seminal Plasma by Using Raman Spectroscopy," Appl. Spectrosc. 67, 757-760 (2013)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-67-7-757

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