OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 3 — Mar. 6, 2014

Infrared and Raman Imaging for Characterizing Complex Biological Materials: A Comparative Morpho-Spectroscopic Study of Colon Tissue

Jayakrupakar Nallala, Olivier Piot, Marie-Danièle Diebold, Cyril Gobinet, Olivier Bouché, Michel Manfait, and Ganesh D. Sockalingum

Applied Spectroscopy, Vol. 68, Issue 1, pp. 57-68 (2014)


View Full Text Article

Acrobat PDF (1222 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations
  • Export Citation/Save Click for help

Abstract

Complementary diagnostic methods to conventional histopathology are currently being investigated for developing rapid and objective molecular-level understanding of various disorders, especially cancers. Spectral histopathology using vibrational spectroscopic imaging has been put in the frontline as potentially promising in this regard as it provides a “spectral fingerprint” of the biochemical composition of cells and tissues. In order to ascertain the feasible conditions of vibrational spectroscopic methods for tissue-imaging analysis, vibrational multimodal imaging (infrared transmission, infrared-attenuated total reflection, and Raman imaging) of the same colon tissue has been implemented. The spectral images acquired were subjected to multivariate clustering analysis in order to identify on a molecular level the constituent histological organization of the colon tissue such as the epithelium, connective tissue, etc., by comparing the cluster images with the histological reference images. Based on this study, a comparative analysis of important factors involved in the vibrational multimodal imaging approaches such as image resolution, time constraints, their advantages and limitations, and their applicability to biological tissues has been carried out. Out of the three different vibrational imaging modalities tested, infrared-attenuated total reflection mode of imaging appears to provide a good compromise between the tissue histology and the time constraints in achieving similar image contrast to that of Raman imaging at an approximately 33-fold faster measurement time. The present study demonstrates the advantages, the limitations of the important parameters involved in vibrational multimodal imaging approaches, and their potential application toward imaging of biological tissues.

Virtual Issues
Vol. 9, Iss. 3 Virtual Journal for Biomedical Optics

Citation
Jayakrupakar Nallala, Olivier Piot, Marie-Danièle Diebold, Cyril Gobinet, Olivier Bouché, Michel Manfait, and Ganesh D. Sockalingum, "Infrared and Raman Imaging for Characterizing Complex Biological Materials: A Comparative Morpho-Spectroscopic Study of Colon Tissue," Appl. Spectrosc. 68, 57-68 (2014)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=as-68-1-57

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited