Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy can be used as a powerful imaging technique to identify chemical compositions of complex samples in biology, biophysics, medicine, and materials science. In this work we developed a CARS microscopic system capable of hyperspectral imaging. By employing an ultrafast laser source, a photonic crystal fiber, and a scanning laser microscope together with spectral detection by a highly sensitive back-illuminated cooled charge-coupled device (CCD) camera, we were able to rapidly acquire and process hyperspectral images of live cells with chemical selectivity. We discuss various aspects of hyperspectral CARS image analysis and demonstrate the use of singular value decomposition methods to characterize the cellular lipid content.

Sparse sampling for fast hyperspectral coherent anti-Stokes Raman scattering imaging

Francesco Masia, Paola Borri, and Wolfgang Langbein
Opt. Express 22(4) 4021-4028 (2014)

Simple approach to one-laser, broadband coherent anti-Stokes Raman scattering microscopy

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Label-free tetra-modal molecular imaging of living cells with CARS, SHG, THG and TSFG (coherent anti-Stokes Raman scattering, second harmonic generation, third harmonic generation and third-order sum frequency generation)

Hiroki Segawa, Masanari Okuno, Hideaki Kano, Philippe Leproux, Vincent Couderc, and Hiro-o Hamaguchi
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Vibrationally resonant imaging of a single living cell by supercontinuum-based multiplex coherent anti-Stokes Raman scattering microspectroscopy

Hideaki Kano and Hiro-o Hamaguchi
Opt. Express 13(4) 1322-1327 (2005)

Quantitative index of arbitrary molar concentration for coherent anti-Stoke Raman scattering (CARS) spectroscopy and microscopy

Hideharu Mikami, Manabu Shiozawa, Masataka Shirai, and Koichi Watanabe
Opt. Express 23(4) 5300-5311 (2015)