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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 9, Iss. 5 — Apr. 29, 2014

New Design Method for a Phase Plate in Super-Resolution Fluorescence Microscopy

Nandor Bokor and Yoshinori Iketaki

Applied Spectroscopy, Vol. 68, Issue 3, pp. 353-361 (2014)

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The performance of a super-resolution fluorescence depletion microscope system depends crucially on the precise alignment of the pump and erase beams with the axis of the focusing objective. Here, we propose a new design method for a two-color spiral phase plate with a single-layer structure (S2SPP), and we experimentally investigate the image properties given by the phase plate. In spite of its simple structure, the plate can provide a super-resolution image with a spatial resolution better than 70 nm. Beside eliminating alignment problems and yielding a compact setup, the simplicity of fabrication of the S2SPP makes our proposed method very suitable for commercial microscope systems.

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Vol. 9, Iss. 5 Virtual Journal for Biomedical Optics

Nandor Bokor and Yoshinori Iketaki, "New Design Method for a Phase Plate in Super-Resolution Fluorescence Microscopy," Appl. Spectrosc. 68, 353-361 (2014)

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