Abstract

Novel optical spectroscopy and imaging methods may be valuable in the early detection of cancer. This paper reports differences in the luminescence responses of pigmented skin lesions (melanomas and nevi) and apparently normal non-pigmented human skin, based on analyses of synchronous luminescence spectroscopy measurements. Measurements were performed in the excitation range of 330-545 nm, with synchronous intervals varying from 30-120 nm. Normal skin, nevi, and melanomas differ in the way they fluoresce, and these differences are more distinct in the synchronous fluorescence spectra than in the conventional emission and excitation spectra. The differences in the fluorescence characteristics of pigmented and normal skin samples were ascribed to differences in concentrations of endogenous fluorophores and chromophores. Principal component and linear discriminant analysis of the synchronous spectra measured at different synchronous intervals showed that the greatest variance among the sample groups was at the 70 nm interval spectra. These spectra were then used to create partial least squares discriminant analysis-based classification models. Evaluation of the quality of these models from the receiver operating characteristic curves showed they performed well, with a maximum value of 1 for the area under the curve for melanoma detection. Hence, synchronous luminescence spectroscopy coupled with statistical methods may be advantageous in the early detection of skin cancer.

Differentiation of cutaneous melanoma from surrounding skin using laser-induced breakdown spectroscopy

Jung Hyun Han, Youngmin Moon, Jong Jin Lee, Sujeong Choi, Yong-Chul Kim, and Sungho Jeong
Biomed. Opt. Express 7(1) 57-66 (2016)

Clinical evaluation of melanomas and common nevi by spectral imaging

Ilze Diebele, Ilona Kuzmina, Alexey Lihachev, Janis Kapostinsh, Alexander Derjabo, Lauma Valeine, and Janis Spigulis
Biomed. Opt. Express 3(3) 467-472 (2012)

Time-resolved fluorescence lifetime for cutaneous melanoma detection

Layla Pires, Marcelo Saito Nogueira, Sebastião Pratavieira, Lilian Tan Moriyama, and Cristina Kurachi
Biomed. Opt. Express 5(9) 3080-3089 (2014)

Differentiation of seborrheic keratosis from basal cell carcinoma, nevi and melanoma by RGB autofluorescence imaging

Alexey Lihachev, Ilze Lihacova, Emilija V. Plorina, Marta Lange, Alexander Derjabo, and Janis Spigulis
Biomed. Opt. Express 9(4) 1852-1858 (2018)

Comparison of photoacoustic imaging and histopathological examination in determining the dimensions of 52 human melanomas and nevi ex vivo

Jenny Hult, Aboma Merdasa, Agnes Pekar-Lukacs, Magne Tordengren Stridh, Azin Khodaverdi, John Albinsson, Bodil Gesslein, Ulf Dahlstrand, Linn Engqvist, Yousef Hamid, Douglas Larsson Albèr, Bertil Persson, Tobias Erlöv, Rafi Sheikh, Magnus Cinthio, and Malin Malmsjö
Biomed. Opt. Express 12(7) 4097-4114 (2021)