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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 6 — Jun. 13, 2007

Photoreceptor counting and montaging of en-face retinal images from an adaptive optics fundus camera

Bai Xue, Stacey S. Choi, Nathan Doble, and John S. Werner  »View Author Affiliations


JOSA A, Vol. 24, Issue 5, pp. 1364-1372 (2007)
http://dx.doi.org/10.1364/JOSAA.24.001364


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Abstract

A fast and efficient method for quantifying photoreceptor density in images obtained with an en-face flood-illuminated adaptive optics (AO) imaging system is described. To improve accuracy of cone counting, en-face images are analyzed over extended areas. This is achieved with two separate semiautomated algorithms: (1) a montaging algorithm that joins retinal images with overlapping common features without edge effects and (2) a cone density measurement algorithm that counts the individual cones in the montaged image. The accuracy of the cone density measurement algorithm is high, with > 97 % agreement for a simulated retinal image (of known density, with low contrast) and for AO images from normal eyes when compared with previously reported histological data. Our algorithms do not require spatial regularity in cone packing and are, therefore, useful for counting cones in diseased retinas, as demonstrated for eyes with Stargardt’s macular dystrophy and retinitis pigmentosa.

© 2007 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(100.2000) Image processing : Digital image processing
(100.2960) Image processing : Image analysis
(330.5310) Vision, color, and visual optics : Vision - photoreceptors

ToC Category:
Image Processing

History
Original Manuscript: August 15, 2006
Revised Manuscript: November 10, 2006
Manuscript Accepted: December 1, 2006
Published: April 11, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Bai Xue, Stacey S. Choi, Nathan Doble, and John S. Werner, "Photoreceptor counting and montaging of en-face retinal images from an adaptive optics fundus camera," J. Opt. Soc. Am. A 24, 1364-1372 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-24-5-1364


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