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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Scattering polarization by anisotropic biomolecules

Tsu-Wei Nee, Soe-Mie F. Nee, De-Ming Yang, and Yu-Shan Huang  »View Author Affiliations


JOSA A, Vol. 25, Issue 5, pp. 1030-1038 (2008)
http://dx.doi.org/10.1364/JOSAA.25.001030


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Abstract

The full polarization properties of anisotropic biomolecule optical scattering are investigated theoretically. By using a simple ellipsoid model of a single biomolecule, the scattering fields and Mueller matrices are derived from fundamental electromagnetism theory. The energy of scattered photons is not necessarily equal to that of the incident laser beam. This theory can be generally applied to the experiments of fluorescence, Raman scattering, and second-harmonic generation. Fitting of a single tetramethylrhodamine-labeled lipid molecule’s anisotropic imaging experiment is demonstrated. This theory has provided a fundamental simulation analysis tool of understanding and developing the optical polarimetric sensing science and technology of the anisotropic biomolecules and biomedium. The medium dielectric constant of the model ellipsoid provides a theoretic background for correlating the optical polarization properties of a biomolecule to its microscopic electronic structure.

© 2008 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(290.1350) Scattering : Backscattering
(290.5840) Scattering : Scattering, molecules
(290.5825) Scattering : Scattering theory
(290.5855) Scattering : Scattering, polarization

ToC Category:
Scattering

History
Original Manuscript: January 8, 2008
Manuscript Accepted: February 22, 2008
Published: April 15, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Tsu-Wei Nee, Soe-Mie F. Nee, De-Ming Yang, and Yu-Shan Huang, "Scattering polarization by anisotropic biomolecules," J. Opt. Soc. Am. A 25, 1030-1038 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-25-5-1030

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