Abstract

Inverse light scattering analysis seeks to associate measured scattering properties with the most probable theoretical scattering distribution. Although Mie theory is a spherical scattering model, it has been used successfully for discerning the geometry of spheroidal scatterers. The goal of this study was an in-depth evaluation of the consequences of analyzing the structure of spheroidal geometries, which are relevant to cell and tissue studies in biology, by employing Mie-theory-based inverse light scattering analysis. As a basis for this study, the scattering from spheroidal geometries was modeled using T-matrix theory and used as test data. In a previous study, we used this technique to investigate the case of spheroidal scatterers aligned with the optical axis. In the present study, we look at a broader scope which includes the effects of aspect ratio, orientation, refractive index, and incident light polarization. Over this wide range of parameters, our results indicate that this method provides a good estimate of spheroidal structure.

© 2008 Optical Society of America

Experimental verification of T-matrix-based inverse light scattering analysis for assessing structure of spheroids as models of cell nuclei

Cyrus Amoozegar, Michael G. Giacomelli, Justin D. Keener, Kevin J. Chalut, and Adam Wax
Appl. Opt. 48(10) D20-D25 (2009)

Application of Mie theory to determine the structure of spheroidal scatterers in biological materials

Justin D. Keener, Kevin J. Chalut, John W. Pyhtila, and Adam Wax
Opt. Lett. 32(10) 1326-1328 (2007)

Evaluation of hybrid algorithm for analysis of scattered light using ex vivo nuclear morphology measurements of cervical epithelium

Derek Ho, Tyler K. Drake, Rex C. Bentley, Fidel A. Valea, and Adam Wax
Biomed. Opt. Express 6(8) 2755-2765 (2015)

Application of the T-matrix method to determine the structure of spheroidal cell nuclei with angle-resolved light scattering

Michael G. Giacomelli, Kevin J. Chalut, Julie H. Ostrander, and Adam Wax
Opt. Lett. 33(21) 2452-2454 (2008)

Size and shape determination of spheroidal scatterers using two-dimensional angle resolved scattering

Michael Giacomelli, Yizheng Zhu, John Lee, and Adam Wax
Opt. Express 18(14) 14616-14626 (2010)