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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 4 — Apr. 1, 2009

Scanning holographic microscopy with spatially incoherent sources: reconciling the holographic advantage with the sectioning advantage

Guy Indebetouw  »View Author Affiliations

JOSA A, Vol. 26, Issue 2, pp. 252-258 (2009)

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A new mode of operation of scanning holographic microscopy that combines the two seemingly incompatible holographic advantage and axial sectioning advantage is proposed and demonstrated. Temporally modulated Fabry–Perot fringes are scanned in a two-dimensional raster over the specimen. With a spatially coherent (point) source, the fringes are spatially nonlocalized and encode the entire three-dimensional volume of the specimen in the form of a single-sideband in-line Fresnel hologram. With an extended spatially incoherent source the fringes are axially localized and select the modulated information from a specific axial plane only. The holographic advantage and the sectioning advantage can thus be achieved with the same scanning holographic setup using different source sizes.

© 2009 Optical Society of America

OCIS Codes
(110.6880) Imaging systems : Three-dimensional image acquisition
(180.0180) Microscopy : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: August 7, 2008
Revised Manuscript: November 14, 2008
Manuscript Accepted: December 4, 2008
Published: January 20, 2009

Virtual Issues
Vol. 4, Iss. 4 Virtual Journal for Biomedical Optics

Guy Indebetouw, "Scanning holographic microscopy with spatially incoherent sources: reconciling the holographic advantage with the sectioning advantage," J. Opt. Soc. Am. A 26, 252-258 (2009)

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