Abstract
A new integration based fluorescence lifetime imaging microscopy (FLIM) called IEM has been proposed to implement lifetime extraction [J. Opt. Soc. Am. A 25, 1190 (2008) ]. A real-time hardware implementation of the IEM FLIM algorithm suitable for single photon avalanche diode arrays in nanometer-scale CMOS technology is now proposed. The problems of reduced pixel readout bandwidth and background noise are studied and a calibration method suitable for FPGA implementation is introduced. In particular, the relationship between signal-to-noise ratio and background noise is considered based on statistics theory and compared with a rapid lifetime determination method and maximum-likelihood estimator with–without background correction. The results are also compared with Monte Carlo simulations giving good agreement. The performance of the proposed methods has been tested on monoexponential decay experimental data. The high flexibility, wide range, and hardware friendliness make IEM the best candidate for system-on-chip integration to our knowledge.
© 2009 Optical Society of America
Full Article | PDF ArticleMore Like This
Day-Uei Li, Jochen Arlt, Justin Richardson, Richard Walker, Alex Buts, David Stoppa, Edoardo Charbon, and Robert Henderson
Opt. Express 18(10) 10257-10269 (2010)
Day-Uei Li, Eleanor Bonnist, David Renshaw, and Robert Henderson
J. Opt. Soc. Am. A 25(5) 1190-1198 (2008)
Simon P. Poland, Ahmet T. Erdogan, Nikola Krstajić, James Levitt, Viviane Devauges, Richard J. Walker, David Day-Uei Li, Simon M. Ameer-Beg, and Robert K. Henderson
Opt. Express 24(7) 6899-6915 (2016)