Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Modeling phase microscopy of transparent three-dimensional objects: a product-of-convolutions approach

Not Accessible

Your library or personal account may give you access

Abstract

We present a product-of-convolutions (POC) model for phase microscopy images. The model was designed to simulate phase images of thick heterogeneous transparent objects. The POC approach attempts to capture phase delays along the optical axis by modeling the imaged object as a stack of parallel slices. The product of two-dimensional convolutions between each slice and the appropriate slice of the point spread function is used to represent the object at the image plane. The total electric field at the image plane is calculated as the product of the object function and the incident field. Phase images from forward models based on the first Born and Rytov approximations are used for comparison. Computer simulations and measured images illustrate the ability of the POC model to represent phase images from thick heterogeneous objects accurately, cases where the first Born and Rytov models have well-known limitations. Finally, measured phase microscopy images of mouse embryos are compared to those produced by the Born, Rytov, and POC models. Our comparisons show that the POC model is capable of producing accurate representations of these more complex phase images.

© 2009 Optical Society of America

Full Article  |  PDF Article
More Like This
Image formation of thick three-dimensional objects in differential-interference-contrast microscopy

Sigal Trattner, Eugene Kashdan, Micha Feigin, and Nir Sochen
J. Opt. Soc. Am. A 31(5) 968-980 (2014)

Validity criterion for the Born approximation convergence in microscopy imaging

Sigal Trattner, Micha Feigin, Hayit Greenspan, and Nir Sochen
J. Opt. Soc. Am. A 26(5) 1147-1156 (2009)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (6)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (17)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved