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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 8 — Jul. 30, 2009

Closed-form representations of field components of fluorescent emitters in layered media

Mehmet Dogan, M. Irsadi Aksun, Anna K. Swan, Bennett B. Goldberg, and M. Selim Ünlü  »View Author Affiliations


JOSA A, Vol. 26, Issue 6, pp. 1458-1466 (2009)
http://dx.doi.org/10.1364/JOSAA.26.001458


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Abstract

Dipole radiation in and near planar stratified dielectric media is studied theoretically within the context of fluorescence microscopy, as fluorescent emitters are generally modeled by electric dipoles. Although the main emphasis of this study is placed on the closed-form representations of the field components of fluorescent emitters in layered environments in near- and far-field regions, the underlying motive is to understand the limits of spectral self-interference fluorescence microscopy in studying the dipole orientation of fluorophores. Since accurate calculations of the field components of arbitrarily polarized electric dipoles in layered environments are computationally very time-consuming, a method for finding their closed-form representations is proposed using the closed-form potential Green’s functions previously developed for microwave applications. The method is verified on typical geometries used in spectral self-interference microscopy experiments, where a dipole emitter is positioned over a slab of Si O 2 on top of a Si substrate. In addition to facilitating efficient calculation of near and intermediate fields of fluorescent emitters, closed-form Green’s functions for fields would also play a crucial role in developing efficient and rigorous computational analysis and design tools for optical passive devices such as optical antennas by significantly improving the computational cost of the numerical solution of the integral equation.

© 2009 Optical Society of America

OCIS Codes
(260.2110) Physical optics : Electromagnetic optics
(260.2510) Physical optics : Fluorescence
(260.3160) Physical optics : Interference
(350.5500) Other areas of optics : Propagation
(350.5610) Other areas of optics : Radiation

ToC Category:
Physical Optics

History
Original Manuscript: October 21, 2008
Revised Manuscript: April 11, 2009
Manuscript Accepted: April 13, 2009
Published: May 27, 2009

Virtual Issues
Vol. 4, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Mehmet Dogan, M. Irsadi Aksun, Anna K. Swan, Bennett B. Goldberg, and M. Selim Ünlü, "Closed-form representations of field components of fluorescent emitters in layered media," J. Opt. Soc. Am. A 26, 1458-1466 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-26-6-1458

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