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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 4, Iss. 9 — Sep. 4, 2009

Stochastic transfer function for structured illumination microscopy

Michael G. Somekh, Ken Hsu, and Mark C. Pitter  »View Author Affiliations


JOSA A, Vol. 26, Issue 7, pp. 1630-1637 (2009)
http://dx.doi.org/10.1364/JOSAA.26.001630


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Abstract

The stochastic transfer function extends the concept of the conventional transfer function by incorporating noise statistics, thus giving a measure of the signal-to-noise ratio at each spatial frequency. This provides a convenient and standardized metric to assess the trade-off in terms of spatial frequency bandwidth and signal-to-noise ratio for a diverse range of resolution enhancement techniques. We apply this concept to structured illumination microscopy and compare its noise performance as a function of frequency with the conventional wide-field fluorescent microscope. The result suggests how a hybrid algorithm further improves the photon efficiency of the structured illumination technique.

© 2009 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(110.4280) Imaging systems : Noise in imaging systems
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: February 10, 2009
Manuscript Accepted: April 25, 2009
Published: June 19, 2009

Virtual Issues
Vol. 4, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Michael G. Somekh, Ken Hsu, and Mark C. Pitter, "Stochastic transfer function for structured illumination microscopy," J. Opt. Soc. Am. A 26, 1630-1637 (2009)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-26-7-1630


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References

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