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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 1 — Jan. 3, 2011

Multiplicative and subtractive focal volume engineering in coherent Raman microscopy

Varun Raghunathan and Eric Olaf Potma  »View Author Affiliations


JOSA A, Vol. 27, Issue 11, pp. 2365-2374 (2010)
http://dx.doi.org/10.1364/JOSAA.27.002365


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Abstract

Rigorous calculations are performed to study the effective reduction of the nonlinear excitation volumes when using phase-only masks to condition the pump and Stokes driving fields. Focal volume reduction was achieved using both a multiplicative operation of the excitation fields as well as a subtractive operation. Using a tunable optical bottle beam for the Stokes field, an effective reduction of the width of the excitation volume by a factor of 1.5 can be achieved in the focal plane. Further reduction of the focal volume introduces a rapid growth of sidelobes, which renders such volumes unsuitable for imaging applications. In addition, phase sensitive detection was found to provide information from selective sub-divisions of the engineered coherent anti-Stokes Raman scattering excitation volume. In the case of isolated nanoparticles, an apparent resolution improvement by a factor of 3 is demonstrated, and it is shown that the size of sub-diffraction-limited particles can be accurately determined using phase sensitive detection.

© 2010 Optical Society of America

OCIS Codes
(140.3300) Lasers and laser optics : Laser beam shaping
(180.5810) Microscopy : Scanning microscopy
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(270.1670) Quantum optics : Coherent optical effects

ToC Category:
Microscopy

History
Original Manuscript: July 26, 2010
Revised Manuscript: August 31, 2010
Manuscript Accepted: September 1, 2010
Published: October 11, 2010

Virtual Issues
Vol. 6, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Varun Raghunathan and Eric Olaf Potma, "Multiplicative and subtractive focal volume engineering in coherent Raman microscopy," J. Opt. Soc. Am. A 27, 2365-2374 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-27-11-2365

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