Optimized extended depth of focus simulated analysis for confocal microscopy
JOSA A, Vol. 27, Issue 6, pp. 1378-1384 (2010)
http://dx.doi.org/10.1364/JOSAA.27.001378
Enhanced HTML 
Acrobat PDF (547 KB)
Abstract
In imaging systems and especially in confocal microscopy systems there is a trade-off between the lateral resolution and the obtained depth of focus. The use of complex pupils to improve the lateral resolution by engineering the point spread function is a common approach; however the lateral improvement reduces the effective depth of focus and therefore the fluorescence efficiency. In this work we analytically develop an optimized approach for obtaining a complex pupil with an extended depth of focus. The proposed solution is numerically applied and tested in designing an improved focal depth in confocal microscope configuration.
© 2010 Optical Society of America
OCIS Codes
(110.0180) Imaging systems : Microscopy
(140.3300) Lasers and laser optics : Laser beam shaping
(110.2945) Imaging systems : Illumination design
ToC Category:
Imaging Systems
History
Original Manuscript: December 17, 2009
Revised Manuscript: March 29, 2010
Manuscript Accepted: April 6, 2010
Published: May 19, 2010
Virtual Issues
Vol. 5, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Hamootal Duadi, Ofer Margalit, Zeev Zalevsky, and Vassilios Sarafis, "Optimized extended depth of focus simulated analysis for confocal
microscopy," J. Opt. Soc. Am. A 27, 1378-1384 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-27-6-1378
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Generation of a beam with a dark focus surrounded by regions of higher intensity: the optical bottle beam (OL)
- Imaging through scattering media with depth resolution by use of low-coherence gating in spatiotemporal digital holography (OL)
- Microscopy refocusing and dark-field imaging by using a simple LED array (OL)
- Using a quartz paraboloid for versatile wide-field TIR microscopy with sub-nanometer localization accuracy (OE)
- Self-reconstructing sectioned Bessel beams offer submicron optical sectioning for large fields of view in light-sheet microscopy (OE)
Related Conference Papers
- Aperture Coding for Three Dimensional Beamforming
- Computing the 3-D Structure of Viruses from Electron Microscope Images
- Novel Stable Resonator Concept for Efficient Axisymmetric Output from Lasers with a Planar Gain Medium
- Generation of High Order Bessel Beams with Whispering Gallery Mode Resonators
- Highly Efficient Phase-Conjugation of a Pico-Second 1µm Laguerre-Gaussian Beam
« Previous Article | Next Article »
OSA is a member of 