Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Lateral superresolution using a posteriori phase shift estimation for a moving object: experimental results

Not Accessible

Your library or personal account may give you access

Abstract

Structured illumination imaging uses multiple images of an object having different phase shifts in the sinusoidally patterned illumination to obtain lateral superresolution in stationary specimens in microscopy. In our recent work we have discussed a method to estimate these phase shifts a posteriori, allowing us to apply this technique to non-stationary objects such as in vivo tissue. Here we show experimental verification of our earlier simulations for phase shift estimation a posteriori. We estimated phase shifts in fluorescence microscopy images for an object having unknown, random translational motion and used them to obtain an artifact-free reconstruction having the expected superresolution.

© 2010 Optical Society of America

Full Article  |  PDF Article
More Like This
Phase-shift estimation in sinusoidally illuminated images for lateral superresolution

Sapna A. Shroff, James R. Fienup, and David R. Williams
J. Opt. Soc. Am. A 26(2) 413-424 (2009)

Analysis of the CLEAN algorithm and implications for superresolution

David L. Fried
J. Opt. Soc. Am. A 12(5) 853-860 (1995)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (8)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (36)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved