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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 5, Iss. 12 — Sep. 30, 2010

Lateral superresolution using a posteriori phase shift estimation for a moving object: experimental results

Sapna A. Shroff, James R. Fienup, and David R. Williams  »View Author Affiliations


JOSA A, Vol. 27, Issue 8, pp. 1770-1782 (2010)
http://dx.doi.org/10.1364/JOSAA.27.001770


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Abstract

Structured illumination imaging uses multiple images of an object having different phase shifts in the sinusoidally patterned illumination to obtain lateral superresolution in stationary specimens in microscopy. In our recent work we have discussed a method to estimate these phase shifts a posteriori, allowing us to apply this technique to non-stationary objects such as in vivo tissue. Here we show experimental verification of our earlier simulations for phase shift estimation a posteriori. We estimated phase shifts in fluorescence microscopy images for an object having unknown, random translational motion and used them to obtain an artifact-free reconstruction having the expected superresolution.

© 2010 Optical Society of America

OCIS Codes
(050.5080) Diffraction and gratings : Phase shift
(070.0070) Fourier optics and signal processing : Fourier optics and signal processing
(100.3020) Image processing : Image reconstruction-restoration
(100.6640) Image processing : Superresolution
(110.4850) Imaging systems : Optical transfer functions
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:
Image Processing

History
Original Manuscript: January 29, 2010
Revised Manuscript: June 8, 2010
Manuscript Accepted: June 9, 2010
Published: July 9, 2010

Virtual Issues
Vol. 5, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Sapna A. Shroff, James R. Fienup, and David R. Williams, "Lateral superresolution using a posteriori phase shift estimation for a moving object: experimental results," J. Opt. Soc. Am. A 27, 1770-1782 (2010)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-27-8-1770

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