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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 6, Iss. 4 — May. 4, 2011

Parallel two-step phase-shifting digital holograph microscopy based on a grating pair

Peng Gao, Baoli Yao, Irina Harder, Junwei Min, Rongli Guo, Juanjuan Zheng, and Tong Ye  »View Author Affiliations


JOSA A, Vol. 28, Issue 3, pp. 434-440 (2011)
http://dx.doi.org/10.1364/JOSAA.28.000434


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Abstract

An optical configuration for parallel two-step phase-shifting digital holographic microscopy (DHM) based on a grating pair is proposed for the purpose of real-time phase microscopy. Orthogonally circularly polarized object and reference waves are diffracted twice by a pair of gratings, and two parallel copies for each beams come into being. Combined with polarization elements, parallel two-step phase-shifting holograms are obtained. Based on the proposed configuration, two schemes of DHM, i.e., slightly off-axis and on-axis DHM, have been implemented. The slightly off-axis DHM suppresses the dc term by subtracting the two phase-shifting holograms from each other, thus the requirement on the off-axis angle and sampling power of the CCD camera is reduced greatly. The on-axis DHM has the least requirement on the resolving power of the CCD camera, while it requires that the reference wave is premeasured and its intensity is no less than 2 times the maximal intensity of the object wave.

© 2011 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:
Microscopy

History
Original Manuscript: September 30, 2010
Revised Manuscript: December 19, 2010
Manuscript Accepted: December 29, 2010
Published: February 25, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Peng Gao, Baoli Yao, Irina Harder, Junwei Min, Rongli Guo, Juanjuan Zheng, and Tong Ye, "Parallel two-step phase-shifting digital holograph microscopy based on a grating pair," J. Opt. Soc. Am. A 28, 434-440 (2011)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-28-3-434

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