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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 6 — May. 25, 2012

High topographical accuracy by optical shot noise reduction in digital holographic microscopy

Miguel León-Rodríguez, Ramón Rodríguez-Vera, Juan A. Rayas, and Sergio Calixto  »View Author Affiliations


JOSA A, Vol. 29, Issue 4, pp. 498-506 (2012)
http://dx.doi.org/10.1364/JOSAA.29.000498


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Abstract

In this work, we present a new method to reduce the shot noise in phase imaging of digital holograms. A spatial averaging process of phase images reconstructed at different reconstruction distances is performed, with the reconstruction distance range being specified by the numerical focus depth of the optical system. An improved phase image is attained with a 50% shot noise reduction. We use the integral of the angular spectrum as a reconstruction method to obtain a single-object complex amplitude that is needed to perform our proposal. We also show the corresponding simulations and experimental results. The topography of a homemade TiO2 stepwise of 100 nm high was measured and compared with the atomic force microscope results.

© 2012 Optical Society of America

OCIS Codes
(030.4280) Coherence and statistical optics : Noise in imaging systems
(110.0180) Imaging systems : Microscopy
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(120.6660) Instrumentation, measurement, and metrology : Surface measurements, roughness
(090.1995) Holography : Digital holography
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Holography

History
Original Manuscript: September 1, 2011
Revised Manuscript: December 13, 2011
Manuscript Accepted: December 16, 2011
Published: March 16, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Miguel León-Rodríguez, Ramón Rodríguez-Vera, Juan A. Rayas, and Sergio Calixto, "High topographical accuracy by optical shot noise reduction in digital holographic microscopy," J. Opt. Soc. Am. A 29, 498-506 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josaa-29-4-498

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