We report on an experimental setup for single-particle orbit tracking, which allows following fluorescent nanoparticles for more than 10 min with a temporal resolution of 4 ms and a dynamic position accuracy of better than 10 nm. On a model sample—20 nm sized fluorescent polymer beads in glycerol—we will illustrate how artifacts caused by unavoidable experimental shortcomings (might) obscure the experimental result and how misinterpretations can be prevented.
© 2012 Optical Society of America
Original Manuscript: March 16, 2012
Manuscript Accepted: March 30, 2012
Published: June 7, 2012
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics
Dominique Ernst, Stefan Hain, and Jürgen Köhler, "Setup for single-particle orbit tracking: artifacts and corrections," J. Opt. Soc. Am. A 29, 1277-1287 (2012)