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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 6 — Jun. 13, 2007

Shaper-assisted collinear SPIDER: fast and simple broadband pulse compression in nonlinear microscopy

Bernhard von Vacano, Tiago Buckup, and Marcus Motzkus  »View Author Affiliations


JOSA B, Vol. 24, Issue 5, pp. 1091-1100 (2007)
http://dx.doi.org/10.1364/JOSAB.24.001091


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Abstract

In situ characterization and control of the phase of broadband femtosecond pulses in microscopy can be achieved with a novel simplified scheme based on spectral shear interferometry for direct electric field reconstruction (SPIDER): the use of a femtosecond pulse shaper eliminates the need for an interferometer setup, allows dispersion-free SPIDER operation and at the same time compression even of complex pulses. Beyond compression, the scheme allows precise phase control at the site of the microscopic experiment. We present the underlying principles, design considerations, and details of the experimental implementation, and show the successful operation of the shaper-assisted collinear (SAC) SPIDER to characterize, compress, and tailor broadband femtosecond pulses in situ. The reliability is demonstrated by comparison with independent cross-frequency-resolved optical gating measurement, and improved multiphoton imaging with SAC-SPIDER-compressed pulses is shown. Its simplicity and versatility make SAC-SPIDER an extremely useful tool for next-generation broadband nonlinear microscopy.

© 2007 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.0180) Microscopy : Microscopy
(320.5520) Ultrafast optics : Pulse compression
(320.5540) Ultrafast optics : Pulse shaping

ToC Category:
Microscopy

History
Original Manuscript: October 25, 2006
Manuscript Accepted: January 3, 2007
Published: April 17, 2007

Virtual Issues
Vol. 2, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Bernhard von Vacano, Tiago Buckup, and Marcus Motzkus, "Shaper-assisted collinear SPIDER: fast and simple broadband pulse compression in nonlinear microscopy," J. Opt. Soc. Am. B 24, 1091-1100 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josab-24-5-1091

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