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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editors: Andrew Dunn and Anthony Durkin
  • Vol. 7, Iss. 8 — Aug. 2, 2012

Polarization-resolved four-wave mixing microscopy for structural imaging in thick tissues

Fabiana Munhoz, Hervé Rigneault, and Sophie Brasselet  »View Author Affiliations


JOSA B, Vol. 29, Issue 6, pp. 1541-1550 (2012)
http://dx.doi.org/10.1364/JOSAB.29.001541


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Abstract

We present a polarimetric analysis of the four-wave mixing (FWM) signal emitted by thick tissues, in order to extract structural information on molecular order and orientation. A careful analysis of the polarization distortions introduced by the birefringence of the sample is conducted for the proper interpretation of the results. FWM, compared to other well-known nonlinear optical methods such as second-harmonic generation, gives access to additional information on the symmetry of the molecular distribution. Furthermore, it brings the advantage of being generated in any kind of sample, even when centrosymmetry is present. The model developed here is applied to thick rat-tail tendon samples, composed essentially of collagen fibers. We show that, once the birefringence of the sample is completely characterized, it is possible to retrieve the even-order components of the molecular orientational distribution up to the fourth order of symmetry.

© 2012 Optical Society of America

OCIS Codes
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(190.4380) Nonlinear optics : Nonlinear optics, four-wave mixing
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Nonlinear Optics

History
Original Manuscript: March 6, 2012
Manuscript Accepted: April 12, 2012
Published: June 1, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics
June 27, 2012 Spotlight on Optics

Citation
Fabiana Munhoz, Hervé Rigneault, and Sophie Brasselet, "Polarization-resolved four-wave mixing microscopy for structural imaging in thick tissues," J. Opt. Soc. Am. B 29, 1541-1550 (2012)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=josab-29-6-1541


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