OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy

Jenifer F. Ojeda, Changan Xie, Yong-Qing Li, Fred E. Bertrand, John Wiley, and Thomas J. McConnell  »View Author Affiliations


Optics Express, Vol. 14, Issue 12, pp. 5385-5393 (2006)
http://dx.doi.org/10.1364/OE.14.005385


View Full Text Article

Enhanced HTML    Acrobat PDF (142 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The ability to identify specific chromosomes with certainty has been established by the development of several cytogenetic techniques based on staining. Here, we report the use of a new optical technique, laser tweezers and Raman spectroscopy (LTRS), to capture and manipulate chromosomes in order to obtain their spectral patterns for molecular analysis without the need for staining. The purpose of this study was to obtain Raman spectroscopy patterns for chromosomes number 1, 2, and 3 and to test if the Raman spectroscopy pattern could be used to distinguish these three chromosomes. In our experiment, optical tweezers were used to capture the individual chromosomes and the Raman spectral patterns were collected for the trapped chromosomes. Then, the captured chromosome was manipulated with the optical tweezers and moved to another chamber through a micro - channel, in which the chromosomes were G-banded for positive identification as chromosome number 1, 2, or 3. Generalized discriminate analysis (GDA) was used to compare the Raman signatures. This analysis revealed that chromosomes 1, 2, and 3 could be distinguished and identified based on their Raman spectra. Development of this approach will lead to more rapid automatic methods for chromosome analysis and identification without the use of prior staining. Moreover, the Raman spectral patterns may lend themselves to more detailed analysis of chromosomal structure than is currently available with standard staining protocols. Such analysis may some day be useful for rapid, automated screening and diagnosis for certain cancers.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.5660) Medical optics and biotechnology : Raman spectroscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 23, 2006
Revised Manuscript: March 27, 2006
Manuscript Accepted: May 9, 2006
Published: June 12, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Jenifer F. Ojeda, Changan Xie, Yong-Qing Li, Fred E. Bertrand, John Wiley, and Thomas J. McConnell, "Chromosomal analysis and identification based on optical tweezers and Raman spectroscopy," Opt. Express 14, 5385-5393 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-12-5385


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. S. L. Gersen and M. B. Keagle, The Principles of Clinical Cytogenetics, 2nd Edn, (Humana Press, Totowa, N.J. 2005). [CrossRef]
  2. R. Aasland, T. J. Gibson, and A. F. Stewart, "The PHD Finger: Implications for chromatin-mediated transcriptional regulation," Trends. Biochem. Sci. 20, 56-59 (1995). [CrossRef] [PubMed]
  3. Younsook Cho, Anthony Griswold, Catherine Campbell and Kyung-Tai Min. "Individual histone deacetylases in Drosophila modulate transcription of distinct genes," Genomics 86, 606-617. [PubMed]
  4. J. D. Rowley, "The critical role of chromosome translocations in human leukemias," Annu. Rev. Genet. 32, 495-519 (1998). [CrossRef]
  5. F. Mitelman, B. Johansson, F. Mertens. "Fusion genes and rearranged genes as a linear function of chromosome aberrations in cancer," Nat Genet. 36, 331-334 (2004). [CrossRef] [PubMed]
  6. T. A. Look, "Oncogenic transcription factors in the human acute leukemias," Science 278, 1059-1064. (1997). [CrossRef] [PubMed]
  7. C. Xie, M. A. Dinno, and Y. Li, "Near-infrared Raman spectroscopy of single optically trapped biological cells," Opt. Lett. 27, 249-251(2002). [CrossRef]
  8. C. Xie and Y. Q. Li, "Confocal micro-Raman spectroscopy of single biological cells using optical trapping and shifted excitation difference techniques," J. Appl. Phys. 93, 2982-2986 (2003). [CrossRef]
  9. K. Ramser, K. Logg, M. Goksor, M. Kall, and D. Hanstorp, "Resonance Raman spectroscopy of optically trapped functional erythrocytes," J. Biomed. Opt. 9, 593-600 (2004). [CrossRef] [PubMed]
  10. C. Xie, Y. Q. Li, W. Tang, R. J. Newton, "Study of dynamical process of heat denaturation in optically trapped single microorganisms by near-infrared Raman spectroscopy," J. Appl. Phys. 94, 6138-6142 (2003). [CrossRef]
  11. C. Xie, J. Mace, M. A. Dinno, Y. Q. Li, W. Tang, R. J. Newton, P. J. Gemperline, "Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy," Anal. Chem. 77, 4390-4397 (2005). [CrossRef] [PubMed]
  12. R. Gessner, C. Winter, P. Rosch, M. Schmitt, R. Petry, W. Kiefer, M. Lankers, J. Popp, "Identification of biotic and abiotic particles by using a combination of optical tweezers and in situ Raman spectroscopy," Chemphyschem. 5, 1159-1170 (2004). [CrossRef] [PubMed]
  13. J. W. Chan, A. P. Esposito, C. E. Talley, C. W. Hollars, S. M. Lane, and T. Huser, "Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy," Anal. Chem. 76, 599-603 (2004). [CrossRef] [PubMed]
  14. J. M. Sanderson and A. D. Ward, "Analysis of liposomal membrane composition using Raman tweezers," Chem Commun (Camb),  7, 1120-1121 (2004). [CrossRef]
  15. M. D. Mannie, T. McConnell, C. A. Xie, Y.Q. Li, "Activation-dependent phases of T cells distinguished by use of optical tweezers and near infrared Raman spectroscopy," J. Immunol. Methods 297, 53-60 (2005). [CrossRef] [PubMed]
  16. C. Xie, D. Chen, Y.Q. Li, "Raman sorting and identification of single living micro-organisms with optical tweezers," Opt. Lett. 30, 1800-1802 (2005). [CrossRef] [PubMed]
  17. J. Th. Bijman, "Optimization of mammalian chromosome suspension preparations employed in a flow cytometric analysis," Cytometry 3, 354-358 (1982). [CrossRef]
  18. D. S. Moore, and G. P. McCabe, Introduction to the practice of statistics, (New York: W.H. Freeman and Company, 2003).
  19. J. Jiang, R. Tsenkova, and Y. Ozaki, "Principal discriminant variate method for classification of multicollinear data: principle and applications," Anal. Sci. 17, 471-474 (2001).
  20. H. Wang, X. Liu, Y. Li, B. Han, L. Lou, and K. Wang. "Isolation of a single rice chromosome by optical micromanipulation," J. Opt. A-Pure.Opt. 6, 89-93 (2004). [CrossRef]
  21. G. J. Puppels, F.F. de Mul, C. Otto, J. Greve, M. Robert-Nicoud, D. J. Arndt-Jovin, and T. M. Jovin, 1990. "Studying single living cells and chromosomes by confocal Raman microspectroscopy," Nature 347, 301-303. [CrossRef] [PubMed]
  22. I. Notingher, S. Verrier, S. Haque, J. M. Polak, and L. L. Hench, "Spectroscopic study of human lung epithelial cells (A549) in culture: living cells versus dead cells," Biopolymers 72, 230-240 (2003). [CrossRef] [PubMed]
  23. W. L. Peticolas, T. W. Patapoff, G. A. Thomas, J. Postlewait, and J. W. Powell, "Laser Raman microscopy of chromosomes in living Eukaryotic cells: DNA polymorphism in vivo," J. Raman Spectrosc. 27, 571-578 (1996). [CrossRef]
  24. C. A. Xie, J. Mace, M. A. Dinno, Y.Q. Li, W. Tang, R.J. Newton, and P.J. Gemperline, "Identification of single bacterial cells in aqueous solution using confocal laser tweezers Raman spectroscopy," Anal. Chem. 77, 4390-4397. [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 
Fig. 4.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited