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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 7 — Jul. 17, 2006

Focus plane detection criteria in digital holography microscopy by amplitude analysis

Frank Dubois, Cédric Schockaert, Natacha Callens, and Catherine Yourassowsky  »View Author Affiliations


Optics Express, Vol. 14, Issue 13, pp. 5895-5908 (2006)
http://dx.doi.org/10.1364/OE.14.005895


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Abstract

We propose and test a focus plane determination method that computes the digital refocus distance of an object investigated by digital holographic microscopy working in transmission. For this purpose we analyze the integrated amplitude modulus as a function of the digital holographic reconstruction distance. It is shown that when the focus distance is reached, the integrated amplitude is minimum for pure amplitude object and maximum for pure phase object. After a theoretical analysis, the method is demonstrated on actual digital holograms for the refocusing of pure amplitude and of pure phase microscopic samples.

© 2006 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(090.2880) Holography : Holographic interferometry
(180.3170) Microscopy : Interference microscopy

ToC Category:
Holography

History
Original Manuscript: May 4, 2006
Revised Manuscript: June 6, 2006
Manuscript Accepted: June 9, 2006
Published: June 26, 2006

Virtual Issues
Vol. 1, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Frank Dubois, Cédric Schockaert, Natcaha Callens, and Catherine Yourassowsky, "Focus plane detection criteria in digital holography microscopy by amplitude analysis," Opt. Express 14, 5895-5908 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-13-5895


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