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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

Background reconstruction for camera-based thin-layer chromatography using the concave distribution feature of illumination

Li Zhang  »View Author Affiliations

Optics Express, Vol. 14, Issue 22, pp. 10386-10392 (2006)

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This paper presents a method to reconstruct the nonuniform background for camera-based quantitative evaluation of thin-layer chromatography (TLC). After analyzing the concave distribution feature of illumination produced by a linear light source on a plane, the paper then makes use of the feature and convex hull algorithm to find points belonging to the background. After that, B-spline is employed to reconstruct the background. An experiment is also made to test the performance of the method, which shows that the correlation coefficient between the linear samples is 0.9949 after removing the estimated background.

© 2006 Optical Society of America

OCIS Codes
(100.0100) Image processing : Image processing
(120.4640) Instrumentation, measurement, and metrology : Optical instruments
(170.3010) Medical optics and biotechnology : Image reconstruction techniques

ToC Category:
Image Processing

Original Manuscript: August 8, 2006
Revised Manuscript: October 4, 2006
Manuscript Accepted: October 9, 2006
Published: October 30, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Li Zhang, "Background reconstruction for camera-based thin-layer chromatography using the concave distribution feature of illumination," Opt. Express 14, 10386-10392 (2006)

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