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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 11 — Nov. 13, 2006

Finesse and sensitivity gain in cavity-enhanced absorption spectroscopy of biomolecules in solution

Timothy McGarvey, André Conjusteau, and Hideo Mabuchi  »View Author Affiliations


Optics Express, Vol. 14, Issue 22, pp. 10441-10451 (2006)
http://dx.doi.org/10.1364/OE.14.010441


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Abstract

We describe a ‘wet mirror’ apparatus for cw cavity-enhanced absorption measurements with Bacteriochlorophyll a (BChla) in solution and show that it achieves the full sensitivity gain (≈ 2.3 × 104) afforded by the finesse (3.4 × 104) and loss distribution of our optical resonator. This result provides an important proof-of-principle demonstration for solution-phase cavity-enhanced spectroscopy; straightforward extrapolation to a system with state-of-the-art low-loss mirrors and shot-noise-limited performance indicates that single molecule sensitivity in liquids is within reach of current technology. With the probe laser locked to the cavity resonance, our instrument achieves a sensitivity ≈ 3.4 × 10-8/√Hz (for a sample of length 1.75 mm) with 100 kHz bandwidth and can reliably detect sub-nM concentrations of BChla with 1 ms integration time.

© 2006 Optical Society of America

OCIS Codes
(120.2230) Instrumentation, measurement, and metrology : Fabry-Perot
(300.6250) Spectroscopy : Spectroscopy, condensed matter

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: September 5, 2006
Revised Manuscript: October 12, 2006
Manuscript Accepted: October 20, 2006
Published: October 30, 2006

Virtual Issues
Vol. 1, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Timothy McGarvey, André Conjusteau, and Hideo Mabuchi, "Finesse and sensitivity gain in cavity-enhanced absorption spectroscopy of biomolecules in solution," Opt. Express 14, 10441-10451 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-22-10441


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