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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 12 — Dec. 18, 2006

Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy

Keisuke Isobe, Yasuyuki Ozeki, Takehito Kawasumi, Shogo Kataoka, Shin’ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh  »View Author Affiliations


Optics Express, Vol. 14, Issue 23, pp. 11204-11214 (2006)
http://dx.doi.org/10.1364/OE.14.011204


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Abstract

We present spectral interferometric four-wave mixing (FWM) microscopy with a nearly shot-noise limited sensitivity and with the capability of separating FWM signals from fluorescence signals. We analyze the requirements for obtaining the shot-noise limited sensitivity and experimentally achieve the sensitivity that is only 4-dB lower than the shotnoise limit. Moreover, we show that only FWM signals can be extracted through the Fourier filtering even when the FWM spectrum is overlapped and overwhelmed by the fluorescence spectrum. We demonstrate simultaneous acquisition of FWM and two-photon excited fluorescence images of fluorescent monodispersed polystyrene microspheres.

© 2006 Optical Society of America

OCIS Codes
(180.6900) Microscopy : Three-dimensional microscopy
(190.4410) Nonlinear optics : Nonlinear optics, parametric processes
(300.1030) Spectroscopy : Absorption
(300.6290) Spectroscopy : Spectroscopy, four-wave mixing
(320.7110) Ultrafast optics : Ultrafast nonlinear optics

ToC Category:
Microscopy

History
Original Manuscript: September 19, 2006
Revised Manuscript: October 22, 2006
Manuscript Accepted: October 24, 2006
Published: November 13, 2006

Virtual Issues
Vol. 1, Iss. 12 Virtual Journal for Biomedical Optics

Citation
Keisuke Isobe, Yasuyuki Ozeki, Takehito Kawasumi, Shogo Kataoka, Shin'ichiro Kajiyama, Kiichi Fukui, and Kazuyoshi Itoh, "Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy," Opt. Express 14, 11204-11214 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-23-11204


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References

  1. R. Hellwarth and P. Christensen, "Nonlinear optical microscopic examination of structure in polycrystalline ZnSe," Opt. Commun. 12, 318-322 (1974). [CrossRef]
  2. J. N. Gannaway and C. J. R. Sheppard, "Second-harmonic imaging in the scanning optical microscope," Opt. and Quant. Elec. 10, 435-439 (1978). [CrossRef]
  3. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third harmonic generation," Appl. Phys. Lett. 70, 922-924 (1997). [CrossRef]
  4. M. Müller, J. Squier, K. R. Wilson, and G. J. Brankenhoff, "3D microscopy of transparent objects using third-harmonic generation," J. Microsc. 191, 266-274 (1998). [CrossRef] [PubMed]
  5. M. D. Duncan, J. Reintjes, and T. J. Manuccia, "Scanning coherent anti-Stokes Raman microscope," Opt. Lett. 7, 350-352 (1982). [CrossRef] [PubMed]
  6. A. Zumbusch, G. R. Holtom and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82, 4142-4145 (1999). [CrossRef]
  7. K. Isobe, S. Kataoka, R. Murase, W. Watanabe, T. Higashi, S. Kawakami, S. Matsunaga, K. Fukui, and K. Itoh, "Stimulated parametric emission microscopy," Opt. Express 14, 786-793 (2006). [CrossRef]
  8. L. Moreaux, O. Sandre, M. Blanchard-Desce, and J. Mertz, "Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy," Opt. Lett. 25, 320-322 (2000). [CrossRef]
  9. A. Zoumi, A. Yeh, and B. J. Tromberg, "Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence," Proc. Natl. Acad. Sci. USA 99, 11014-11019 (2002). [CrossRef] [PubMed]
  10. M. Kobayashi, K. Fujita, O. Nakamura, and S. Kawata, "Time-gated imaging for multifocus second-harmonic generation microscopy," Rev. Sci. Instrum. 76, 073704-1-073704-4 (2005). [CrossRef]
  11. R. K. Chang, J. Ducuing, and N. Bloembergen, "Relative phase measurement between fundamental and second-harmonic light," Phys. Rev. Lett. 15, 6-9 (1965). [CrossRef]
  12. G. Marowsky, and G. Lüpke, "CARS-background suppression by phase-controlled nonlinear interferometry," Appl. Phys. B 51, 49-51 (1990). [CrossRef]
  13. Y. Jiang, I. Tomov, Y. Wang, and Z. Chen, "Second-harmonic optical coherence tomography," Opt. Lett. 29, 1090-1092 (2004). [CrossRef] [PubMed]
  14. C. Vinegoni, J. S. Bredfeldt, and D. L. Marks, "Nonlinear optical contrast enhancement for optical coherence tomography," Opt. Express 12, 331-341 (2004). [CrossRef] [PubMed]
  15. K. Isobe, W. Watanabe, and K. Itoh, "Interferometric second-harmonic-generation microscopy," in Optics Japan 2002 Extended Abstracts (Optical Society of Japan (Japanese Society of Applied Physics), Tokyo, 2002), pp. 18-19.
  16. S. Yazdanfar, L. H. Laiho, and P. T. C. So, "Interferometric second harmonic generation microscopy," Opt. Express 12, 2739-2745 (2004). [CrossRef] [PubMed]
  17. E. O. Potma, C. L. Evans, and X. S. Xie, "Heterodyne coherent anti-Stokes Raman scattering (CARS) imging," Opt. Lett. 31, 241-243 (2006). [CrossRef] [PubMed]
  18. C. L. Evans, E. O. Potma, and X. S. Xie, "Coherent anti-Stokes Raman scattering spectral interferometry: determination of the real and imaginary components of nonlinear susceptibility χ(3) for vibrational microscopy," Opt. Lett. 29, 2923-2925 (2004). [CrossRef]
  19. M. V. Sarunic, B. E. Applegate, and J. A. Izatt, "Spectral domain second-harmonic optical coherence tomography," Opt. Lett. 30, 2391-2393 (2005). [CrossRef] [PubMed]
  20. G. W. H. Wurpel, J. M. Schins and M. Müller, "Direct measurement of chain order in single lipid mono- and bilayers with multiplex CARS," J. Phys. Chem. B 108, 3400-3403 (2004). [CrossRef]
  21. J. F. de Boer, B. Cense, B. H. Park, M. C. Pierce, G. J. Tearney, and B. E. Bouma, "Improved signal-to-noise ratio in spectral-domain compared with time-domain optical coherence tomography," Opt. Lett. 28, 2067-2069 (2003). [CrossRef] [PubMed]
  22. R. Leitgeb, C. K. Hitzenberger, and A. F. Ferchar, "Performance of Fourier domain vs. time domain optical coherence tomography," Opt. Express 11, 889-894 (2003). [CrossRef] [PubMed]
  23. R. Adair, L. L. Chase, and S. A. Payne, "Nonlinear refractive index of optical crystal," Phys. Rev. B 39, 3337-3350 (1989). [CrossRef]
  24. P. T. So, C. Y. Dong, B. R. Masters and K. M. Berland, "Two-photon excitation fluorescence microscopy," Annu. Rev. Biomed. Eng. 2, 399-429 (2000). [CrossRef]

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