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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

Optics InfoBase > Virtual Journal for Biomedical Optics > Volume 2 > Issue 1 > Page 12199

Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment

Ivan R. Perch-Nielsen, Peter John Rodrigo, Carlo Amadeo Alonzo, and Jesper Glückstad  »View Author Affiliations


Optics Express, Vol. 14, Issue 25, pp. 12199-12205 (2006)
http://dx.doi.org/10.1364/OE.14.012199


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Abstract

The Generalized Phase Contrast (GPC) method of optical 3D manipulation has previously been used for controlled spatial manipulation of live biological specimen in real-time. These biological experiments were carried out over a time-span of several hours while an operator intermittently optimized the optical system. Here we present GPC-based optical micromanipulation in a microfluidic system where trapping experiments are computer-automated and thereby capable of running with only limited supervision. The system is able to dynamically detect living yeast cells using a computer-interfaced CCD camera, and respond to this by instantly creating traps at positions of the spotted cells streaming at flow velocities that would be difficult for a human operator to handle. With the added ability to control flow rates, experiments were also carried out to confirm the theoretically predicted axially dependent lateral stiffness of GPC-based optical traps.

© 2006 Optical Society of America

OCIS Codes
(120.1880) Instrumentation, measurement, and metrology : Detection
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 27, 2006
Revised Manuscript: November 29, 2006
Manuscript Accepted: November 30, 2006
Published: December 11, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Ivan R. Perch-Nielsen, Peter John Rodrigo, Carlo Amadeo Alonzo, and Jesper Glückstad, "Autonomous and 3D real-time multi-beam manipulation in a microfluidic environment," Opt. Express 14, 12199-12205 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-25-12199


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References

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