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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

The effect of integration time on fluctuation measurements: calibrating an optical trap in the presence of motion blur

Wesley P. Wong and Ken Halvorsen  »View Author Affiliations

Optics Express, Vol. 14, Issue 25, pp. 12517-12531 (2006)

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Dynamical instrument limitations, such as finite detection bandwidth, do not simply add statistical errors to fluctuation measurements, but can create significant systematic biases that affect the measurement of steady-state properties. Such effects must be considered when calibrating ultra-sensitive force probes by analyzing the observed Brownian fluctuations. In this article, we present a novel method for extracting the true spring constant and diffusion coefficient of a harmonically confined Brownian particle that extends the standard equipartition and power spectrum techniques to account for video-image motion blur. These results are confirmed both numerically with a Brownian dynamics simulation, and experimentally with laser optical tweezers.

© 2006 Optical Society of America

OCIS Codes
(110.4280) Imaging systems : Noise in imaging systems
(140.7010) Lasers and laser optics : Laser trapping
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation

ToC Category:

Original Manuscript: July 21, 2006
Revised Manuscript: October 24, 2006
Manuscript Accepted: November 24, 2006
Published: December 11, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Wesley P. Wong and Ken Halvorsen, "The effect of integration time on fluctuation measurements: calibrating an optical trap in the presence of motion blur," Opt. Express 14, 12517-12531 (2006)

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