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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

Increased optical contrast in imaging of epidermal growth factor receptor using magnetically actuated hybrid gold/iron oxide nanoparticles

Jesse S. Aaron, Junghwan Oh, Timothy A. Larson, Sonia Kumar, Thomas E. Milner, and Konstantin V. Sokolov  »View Author Affiliations

Optics Express, Vol. 14, Issue 26, pp. 12930-12943 (2006)

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We describe a new approach for optical imaging that combines the advantages of molecularly targeted plasmonic nanoparticles and magnetic actuation. This combination is achieved through hybrid nanoparticles with an iron oxide core surrounded by a gold layer. The nanoparticles are targeted in-vitro to epidermal growth factor receptor, a common cancer biomarker. The gold portion resonantly scatters visible light giving a strong optical signal and the superparamagnetic core provides a means to externally modulate the optical signal. The combination of bright plasmon resonance scattering and magnetic actuation produces a dramatic increase in contrast in optical imaging of cells labeled with hybrid gold/iron oxide nanoparticles.

© 2006 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4090) Medical optics and biotechnology : Modulation techniques
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: October 18, 2006
Revised Manuscript: December 2, 2006
Manuscript Accepted: December 7, 2006
Published: December 22, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Jesse S. Aaron, Junghwan Oh, Timothy A. Larson, Sonia Kumar, Thomas E. Milner, and Konstantin V. Sokolov, "Increased optical contrast in imaging of epidermal growth factor receptor using magnetically actuated hybrid gold/iron oxide nanoparticles," Opt. Express 14, 12930-12943 (2006)

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  1. B. N. G. Giepmans, S. R. Adams, M. H. Ellisman, and R. Y. Tsien, "The Fluorescent Toolbox for Assessing Protein Location and Function," Science (Washington, DC, United States) 312, 217-224 (2006). [CrossRef] [PubMed]
  2. D. J. Bornhop, C. H. Contag, K. Licha, and C. J. Murphy, "Advance in contrast agents, reporters, and detection," J. Biomed. Opt. 6, 106-110. (2001). [CrossRef] [PubMed]
  3. J. Malicka, I. Gryczynski, J. Fang, and R. Lakowicz Joseph, "Fluorescence spectral properties of cyanine dye-labeled DNA oligomers on surfaces coated with silver particles," Analytical Biochemistry 317, 136-146. (2003). [CrossRef] [PubMed]
  4. V. Ntziachristos, C.-H. Tung, C. Bremer, and R. Weissleder, "Fluorescence molecular tomography resolves protease activity in vivo," Nature Medicine (New York, NY, United States) 8, 757-761 (2002). [CrossRef] [PubMed]
  5. X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, "In vivo cancer targeting and imaging with semiconductor quantum dots," Nature Biotechnology 22, 969-976 (2004). [CrossRef] [PubMed]
  6. W. C. Chan, and S. Nie, "Quantum dot bioconjugates for ultrasensitive nonisotopic detection," Science 281, 2016-2018. (1998). [CrossRef] [PubMed]
  7. X. Gao, L. Yang, J. A. Petros, F. F. Marshall, J. W. Simons, and S. Nie, "In vivo molecular and cellular imaging with quantum dots," Current Opinion in Biotechnology 16, 63-72 (2005). [CrossRef] [PubMed]
  8. X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Sundaresan, A. M. Wu, S. S. Gambhir, and S. Weiss, "Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics," Science (Washington, DC, United States) 307, 538-544 (2005). [CrossRef] [PubMed]
  9. A. P. Alivisatos, W. Gu, and C. Larabell, "Quantum Dots as Cellular Probes," inAnnual Review of Biomedical Engineering(2005), pp. 55-76.
  10. J. Yguerabide, and E. E. Yguerabide, "Resonance light scattering particles as ultrasensitive labels for detection of analytes in a wide range of applications," Journal of Cellular Biochemistry, 71-81 (2001).
  11. S. J. Oldenburg, R. D. Averitt, S. L. Westcott, and N. J. Halas, "Nanoengineering of optical resonances," Chem. Phys. Lett. 288, 243-247 (1998). [CrossRef]
  12. K. Sokolov, M. Follen, J. Aaron, I. Pavlova, A. Malpica, R. Lotan, and R. Richards-Kortum, "Real-time vital optical imaging of precancer using anti-epidermal growth factor receptor antibodies conjugated to gold nanoparticles," Cancer Research 63, 1999-2004 (2003). [PubMed]
  13. C. Loo, L. Hirsch, M.-H. Lee, E. Chang, J. West, N. Halas, and R. Drezek, "Gold nanoshell bioconjugates for molecular imaging in living cells," Opt. Lett. 30, 1012-1014 (2005). [CrossRef] [PubMed]
  14. I. H. El-Sayed, X. Huang, and M. A. El-Sayed, "Surface plasmon resonance scattering and absorption of anti-EGFR antibody conjugated gold nanoparticles in cancer diagnostics: Applications in oral cancer," Nano Letters 5, 829-834 (2005). [CrossRef] [PubMed]
  15. K. Sokolov, J. Aaron, B. Hsu, D. Nida, A. Gillenwater, M. Follen, C. MacAulay, K. Adler-Storthz, B. Korgel, M. Descour, R. Pasqualini, W. Arap, W. Lam, and R. Richards-Kortum, "Optical systems for in vivo molecular imaging of cancer," Technology in Cancer Research & Treatment 2, 491-504 (2003). [PubMed]
  16. S. V. Kershaw, M. Burt, M. Harrison, A. Rogach, H. Weller, and A. Eychmuller, "Colloidal CdTe/HgTe quantum dots with high photoluminescence quantum efficiency at room temperature," Appl. Phys. Lett. 75, 1694-1696 (1999). [CrossRef]
  17. M. Han, X. Gao, J. Z. Su, and S. Nie, "Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules," Nature Biotechnology 19, 631-635 (2001). [CrossRef] [PubMed]
  18. P. Alivisatos, "The use of nanocrystals in biological detection," Nature Biotechnology 22, 47-52 (2004). [CrossRef] [PubMed]
  19. C. F. Meares, A. J. Chmura, M. S. Orton, T. M. Corneillie, and P. A. Whetstone, "Molecular tools for targeted imaging and therapy of cancer," Journal of Molecular Recognition 16, 255-259 (2003). [CrossRef] [PubMed]
  20. R. Pasqualini, and E. Ruoslahti, "Organ targeting in vivo using phage display peptide libraries," Nature (London) 380, 364-366 (1996). [CrossRef] [PubMed]
  21. P. K. Aravind, A. Nitzan, and H. Metiu, "The interaction between electromagnetic resonances and its role in spectroscopic studies of molecules adsorbed on colloidal particles or metal spheres," Surface Science 110, 189-204 (1981). [CrossRef]
  22. W. Rechberger, A. Hohenau, A. Leitner, J. R. Krenn, B. Lamprecht, and F. R. Aussenegg, "Optical properties of two interacting gold nanoparticles," Opt. Commun. 220, 137-141 (2003). [CrossRef]
  23. R. Elghanian, J. J. Storhoff, R. C. Mucic, R. L. Letsinger, and C. A. Mirkin, "Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles," Science 277, 1078-1080 (1997). [CrossRef] [PubMed]
  24. J. J. Storhoff, A. D. Lucas, V. Garimella, P. Y. Bao, and U. R. Mueller, "Homogeneous detection of unamplified genomic DNA sequences based on colorimetric scatter of gold nanoparticle probes," Nature Biotech. 22, 883-887 (2004). [CrossRef]
  25. C. Soennichsen, B. M. Reinhard, J. Liphardt, and A. P. Alivisatos, "A molecular ruler based on plasmon coupling of single gold and silver nanoparticles," Nature Biotechnol. 23, 741-745 (2005). [CrossRef]
  26. L. Oldenburg Amy, R. Gunther Jillian, and A. Boppart Stephen, "Imaging magnetically labeled cells with magnetomotive optical coherence tomography," Opt. Lett. 30, 747-749. (2005). [CrossRef]
  27. J. N. Anker, and R. Kopelman, "Magnetically modulated optical nanoprobes," Appl. Phys. Lett. 82, 1102-1104 (2003). [CrossRef]
  28. J. Oh, M. Feldman, J. Kim, C. Condit, S. Emelianov, and T. E. Milner, "Detection of magnetic nanoparticles in tissue using magneto-motive ultrasound " Nanotechnology 17, 8 (2006). [CrossRef]
  29. I. Stoeva Savka, F. Huo, J.-S. Lee, and A. Mirkin Chad, "Three-layer composite magnetic nanoparticle probes for DNA," Journal of the American Chemical Society 127, 15362-15363. (2005). [CrossRef]
  30. J. L. Lyon, D. A. Fleming, M. B. Stone, P. Schiffer, and M. E. Williams, "Synthesis of Fe Oxide Core/Au Shell Nanoparticles by Iterative Hydroxylamine Seeding," Nano Letters 4, 719-723 (2004). [CrossRef]
  31. J. Lin, W. Zhou, A. Kumbhar, J. Wiemann, J. Fang, E. E. Carpenter, and C. J. O'Connor, "Gold-Coated Iron (Fe@Au) Nanoparticles: Synthesis, Characterization, and Magnetic Field-Induced Self-Assembly," Journal of Solid State Chemistry 159, 26-31 (2001). [CrossRef]
  32. S.-J. Cho, B. R. Jarrett, A. Y. Louie, and S. M. Kauzlarich, "Gold-coated iron nanoparticles: a novel magnetic resonance agent for T1 and T2 weighted imaging," Nanotechnology 17, 640-644 (2006). [CrossRef]
  33. S.-J. Cho, A. M. Shahin, G. J. Long, J. E. Davies, K. Liu, F. Grandjean, and S. M. Kauzlarich, "Magnetic and Moessbauer Spectral Study of Core/Shell Structured Fe/Au Nanoparticles," Chemistry of Materials 18, 960-967 (2006). [CrossRef]
  34. S. I. Stoeva, F. Huo, J.-S. Lee, and C. A. Mirkin, "Three-Layer Composite Magnetic Nanoparticle Probes for DNA," Journal of the American Chemical Society 127, 15362-15363 (2005). [CrossRef] [PubMed]
  35. H. Wang, D. W. Brandl, F. Le, P. Nordlander, and N. J. Halas, "Nanorice: A Hybrid Plasmonic Nanostructure," Nano Letters 6, 827-832 (2006). [CrossRef] [PubMed]
  36. K. R. Brown, and M. J. Natan, "Hydroxylamine Seeding of Colloidal Au Nanoparticles in Solution and on Surfaces," Langmuir 14, 726-728 (1998). [CrossRef]
  37. J. Jeong, T. H. Ha, and B. H. Chung, "Enhanced reusability of hexa-arginine-tagged esterase immobilized on gold-coated magnetic nanoparticles," Analytica Chimica Acta 569, 203-209 (2006). [CrossRef]
  38. F. H. Wang, I. H. Lee, N. Holmstroem, T. Yoshitake, D. K. Kim, M. Muhammed, J. Frisen, L. Olson, C. Spenger, and J. Kehr, "Magnetic resonance tracking of nanoparticle labelled neural stem cells in a rat's spinal cord," Nanotechnology 17, 1911-1915 (2006). [CrossRef]
  39. W. D. Geoghegan, and G. A. Ackerman, "Adsorption of horseradish peroxidase, ovomucoid and antiimmunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat antihuman immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application," Journal of Histochemistry and Cytochemistry 25, 1187-1200 (1977). [CrossRef] [PubMed]
  40. L. Liu, and H. Elwing, "Complement activation on thiol-modified gold surfaces," Journal of Biomedical Materials Research 30, 535-541 (1996). [CrossRef] [PubMed]
  41. D. Hanahan, and R. A. Weinberg, "The hallmarks of cancer," Cell (Cambridge, Massachusetts) 100, 57-70 (2000). [CrossRef] [PubMed]
  42. A. L. Aden, and M. Kerker, "Scattering of Electromagnetic Waves from Two Concentric Spheres," J. Appl. Phys. 22, 1242-1246 (1951). [CrossRef]
  43. P. B. Johnson, and R. W. Christy, "Optical constants of the noble metals," Physical Review B: Solid State 6, 4370-4379 (1972). [CrossRef]
  44. U. Kreibig, "Properties of Small Particles in Insulating Matrices," in Contribution of Clusters Physics to Material Science and Technology From Isolated Clusters to Aggregated Materials, J. Davenas, and P. M. Rabette, eds. (Klewer Academic Publishers, New York, NY, 1986), pp. 373-423.
  45. D. S. Lidke, P. Nagy, R. Heintzmann, D. J. Arndt-Jovin, J. N. Post, H. E. Grecco, E. A. Jares-Erijman, and T. M. Jovin, "Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction," Nature Biotechnology 22, 198-203 (2004). [CrossRef] [PubMed]
  46. V. Backman, V. Gopal, M. Kalashnikov, K. Badizadegan, R. Gurjar, A. Wax, I. Georgakoudi, M. Mueller, C. W. Boone, R. R. Dasari, and M. S. Feld, "Measuring cellular structure at submicrometer scale with light scattering spectroscopy," IEEE J. Sel. Top. Quantum Electron. 7, 887-893 (2001). [CrossRef]
  47. F. J. Harris, "On the Use of Windows for Harmonic Analysis with the Discrete Fourier Transform," Proceedings of the IEEE 66, 33 (1978). [CrossRef]
  48. T. R. Strick, J. F. Allemand, D. Bensimon, A. Bensimon, and V. Croquette, "The elasticity of a single supercoiled DNA molecule," Science (Washington, D. C.) 271, 1835-1837 (1996). [CrossRef] [PubMed]
  49. C. Wilhelm, F. Gazeau, and J. C. Bacri, "Rotational magnetic endosome microrheology: Viscoelastic architecture inside living cells," Physical Review E: Statistical, Nonlinear, and Soft Matter Physics 67, 061908/061901-061908/061912 (2003). [CrossRef]
  50. H. Huang, J. Sylvan, M. Jonas, R. Barresi, P. T. C. So, K. P. Campbell, and R. T. Lee, "Cell stiffness and receptors: Evidence for cytoskeletal subnetworks," American Journal of Physiology 288, C72-C80 (2005).

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