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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 1 — Jan. 19, 2007

Imaging colloidal particle induced topological defects in a nematic liquid crystal using third harmonic generation microscopy

Rajesh S. Pillai, Masahito Oh-e, Hiroshi Yokoyama, G. J. Brakenhoff, and Michiel Müller  »View Author Affiliations

Optics Express, Vol. 14, Issue 26, pp. 12976-12983 (2006)

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The nature of the third-harmonic generation (THG) process in a nematic liquid crystal is investigated for the case of tightly focused, low intensity, laser beams. Colloidal particle induced topological defects in a liquid crystal are visualized in three-dimensions using the dependence of the THG signal on both changes in non-linear susceptibility and the orientation of the liquid crystal director relative to the incident laser polarization state. We have found that the interpretation of THG images in a liquid crystal is complicated not only by the change in polarisation of the electric field as it propagates through the medium but also by anisotropic refractive index mismatch induced aberrations.

© 2006 Optical Society of America

OCIS Codes
(160.3710) Materials : Liquid crystals
(180.6900) Microscopy : Three-dimensional microscopy
(190.4160) Nonlinear optics : Multiharmonic generation
(190.4410) Nonlinear optics : Nonlinear optics, parametric processes
(190.4720) Nonlinear optics : Optical nonlinearities of condensed matter

ToC Category:
Nonlinear Optics

Original Manuscript: October 6, 2006
Revised Manuscript: November 27, 2006
Manuscript Accepted: November 28, 2006
Published: December 22, 2006

Virtual Issues
Vol. 2, Iss. 1 Virtual Journal for Biomedical Optics

Rajesh S. Pillai, Masahito Oh-e, Hiroshi Yokoyama, G. J. Brakenhoff, and Michiel Müller, "Imaging colloidal particle induced topological defects in a nematic liquid crystal using third harmonic generation microscopy," Opt. Express 14, 12976-12983 (2006)

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  1. M. Schadt, "Liquid crystal materials and liquid crystal displays," Annu. Rev. Mater. Sci. 27, 305-379 (1997). [CrossRef]
  2. A. M. Weiner, "Femtosecond pulse shaping using spatial light modulators," Rev. Sci. Instrum. 71, 1929-1960 (2000). [CrossRef]
  3. I. Musevic, M. Skarabot, U. Tkalec, M. Ravnik, and S. Zumer, "Two-dimensional nematic colloidal crystals self-assembled by topological defects," Science 313, 954-958 (2006). [CrossRef] [PubMed]
  4. P. Poulin, and D. A. Weitz, "Inverted and multiple nematic emulsions," Phys. Rev. E 57, 626 - 637 (1998). [CrossRef]
  5. Y. Gu, and N. L. Abbott, "Observation of saturn-ring defects around solid microspheres in nematic liquid crystals," Phys. Rev. Lett. 85, 4719 - 4722 (2000). [CrossRef] [PubMed]
  6. P. Poulin, H. Stark, T. C. Lubensky, and D. A. Weitz, "Novel colloidal interactions in anisotropic fluids," Science 275, 1770 - 1773 (1997). [CrossRef] [PubMed]
  7. D. Yelin, Y. Silberberg, Y. Barad, and J. S. Patel, "Depth-resolved imaging of nematic liquid crystals by third-harmonic microscopy," Appl. Phys. Lett. 74, 3107-3109 (1999). [CrossRef]
  8. D. Yelin, Y. Silberberg, Y. Barad, and J. S. Patel, "Phase-matched third-harmonic generation in a nematic liquid crystal cell," Phys. Rev. Lett. 82, 3046 - 3049 (1999). [CrossRef]
  9. J.-I. Kosugi, and K. Kajikawaa, "Phase-matched third-harmonic generation from nematic liquid crystals," Appl. Phys. Lett. 84, 5013-5015 (2004). [CrossRef]
  10. R. W. Boyd, Nonlinear optics (Academic Press, Inc., New York, 1992).
  11. J. F. Reintjes, Nonlinear optical parametric processes in liquids and gases (Academic Press, Inc., Orlando, 1984).
  12. T. Tsang, "Optical third-harmonic generation at interfaces," Phys. Rev. A 52, 4116-4125 (1995). [CrossRef] [PubMed]
  13. Y. Barad, H. Eisenberg, M. Horowitz, and Y. Silberberg, "Nonlinear scanning laser microscopy by third-harmonic generation," Appl. Phys. Lett. 70, 922-924 (1997). [CrossRef]
  14. D. Debarre, W. Supatto, A. M. Pena, A. Fabre, T. Tordjmann, L. Combettes, M. C. Schanne-Klein, and E. Beaurepaire, "Imaging lipid bodies in cells and tissues using third-harmonic generation microscopy," Nature Methods 3, 47-53 (2006). [CrossRef]
  15. W. Supatto, D. Débarre, B. Moulia, E. Brouzés, J.-L. Martin, E. Farge, and E. Beaurepaire, "In vivo modulation of morphogenetic movements in Drosophila emryos with femtosecond laser pulses," PNAS 102, 1047-1052 (2005). [CrossRef] [PubMed]
  16. M. Müller, J. Squier, K. R. Wilson, and G. J. Brakenhoff, "3D Microscopy of transparent objects using third-harmonic generation," J. Microsc. 191, 266-274 (1998). [CrossRef] [PubMed]
  17. S.-W. Chu, S.-Y. Chen, T.-H. Tsai, T.-M. Liu, C.-Y. Lin, H.-J. Tsai, and C.-K. Sun, "In vivo developmental biology study using noninvasive multi-harmonic generation microscopy," Opt. Express 11, 3093 - 3099 (2003). [CrossRef] [PubMed]
  18. S.-P. Tai, W.-J. Lee, D.-B. Shieh, P.-C. Wu, H.-Y. Huang, C.-H. Yu, and C.-K. Sun, "In vivo optical biopsy of hamster oral cavity with epi-third-harmonic generation microscopy," Opt. Express 14, 6178-6187 (2006). [CrossRef] [PubMed]
  19. D. Oron, E. Tal, and Y. Silberberg, "Depth-resolved multiphoton polarisation microscopy by third-harmonic generation," Opt. Lett. 28, 2315-2317 (2003). [CrossRef] [PubMed]
  20. R. S. Pillai, G. J. Brakenhoff, and M. Müller, "Analysis of the influence of spherical aberration from focusing through a dielectric slab in quantitative nonlinear optical susceptibility measurements using third-harmonic generation," Opt. Express 14, 260-269 (2006). [CrossRef] [PubMed]

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