Using a novel dual-beam readout with the generalized phase contrast (GPC) method, a multiple-beam 3D real-time micromanipulation system requiring only one spatial light modulator (SLM) has been realized. A theoretical framework for the new GPC scheme with two parallel illumination beams is presented and corroborated with an experimental demonstration. Three-dimensional arrays of polystyrene microbeads were assembled in the newly described system. The use of air immersion objective lenses with GPC-based optical trapping allowed the simultaneous viewing of the assemblies in two orthogonal bright-field imaging perspectives.

© 2006 Optical Society of America

1. A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970). [CrossRef]
2. D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003). [CrossRef] [PubMed]
3. K. Dholakia and P. Reece, "Optical micromanipulation takes hold," Nano Today 1, 18-27 (2006). [CrossRef]
4. P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Real-time three-dimensional optical micromanipulation of multiple particles and living cells," Opt. Lett. 29, 2270-2272 (2004). [CrossRef] [PubMed]
5. P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Four-dimensional optical manipulation of colloidal particles," Appl. Phys. Lett. 86, 074103 (2005). [CrossRef]
6. M. Reicherter, T. Haist, E. U. Wagemann, and H. J. Tiziani, "Optical particle trapping with computer-generated holograms written on a liquid-crystal display," Opt. Lett. 24,608-610 (1999). [CrossRef]
7. G. Sinclair, P. Jordan, J. Courtial, M. Padgett, J. Cooper, and Z. J. Laczik, "Assembly of 3-dimensional structures using programmable holographic optical tweezers," Opt. Express 12,5475-5480 (2004). [CrossRef] [PubMed]
8. J. Glückstad and P. C. Mogensen, "Optimal phase contrast in common-path interferometry," Appl. Opt. 40, 268-282 (2001). [CrossRef]
9. J. W. Goodman, Introduction to Fourier Optics, Second Edition (McGraw-Hill, New York, 1996).
10. I. R. Perch-Nielsen, P. J. Rodrigo, and J. Glückstad, "Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes," Opt. Express 18,2852-2857 (2005). [CrossRef]

Appl. Opt.

• J. Glückstad and P. C. Mogensen, "Optimal phase contrast in common-path interferometry," Appl. Opt. 40, 268-282 (2001). [CrossRef]

Appl. Phys. Lett.

• P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Four-dimensional optical manipulation of colloidal particles," Appl. Phys. Lett. 86, 074103 (2005). [CrossRef]

Nano Today

• K. Dholakia and P. Reece, "Optical micromanipulation takes hold," Nano Today 1, 18-27 (2006). [CrossRef]

Nature

• D. G. Grier, "A revolution in optical manipulation," Nature 424, 810-816 (2003). [CrossRef] [PubMed]

Opt. Express

• G. Sinclair, P. Jordan, J. Courtial, M. Padgett, J. Cooper, and Z. J. Laczik, "Assembly of 3-dimensional structures using programmable holographic optical tweezers," Opt. Express 12,5475-5480 (2004). [CrossRef] [PubMed]
• I. R. Perch-Nielsen, P. J. Rodrigo, and J. Glückstad, "Real-time interactive 3D manipulation of particles viewed in two orthogonal observation planes," Opt. Express 18,2852-2857 (2005). [CrossRef]

Opt. Lett.

• P. J. Rodrigo, V. R. Daria, and J. Glückstad, "Real-time three-dimensional optical micromanipulation of multiple particles and living cells," Opt. Lett. 29, 2270-2272 (2004). [CrossRef] [PubMed]
• M. Reicherter, T. Haist, E. U. Wagemann, and H. J. Tiziani, "Optical particle trapping with computer-generated holograms written on a liquid-crystal display," Opt. Lett. 24,608-610 (1999). [CrossRef]

Phys. Rev. Lett.

• A. Ashkin, "Acceleration and trapping of particles by radiation pressure," Phys. Rev. Lett. 24, 156-159 (1970). [CrossRef]

Other

• J. W. Goodman, Introduction to Fourier Optics, Second Edition (McGraw-Hill, New York, 1996).

Click here to see a list of articles that cite this paper

Related Journal Articles

• Fully dynamic multiple-beam optical tweezers (OE)
• Interactive light-driven and parallel manipulation of inhomogeneous particles (OE)
• Shack-Hartmann multiple-beam optical tweezers (OE)
• Spatial Light Modulator–Controlled Alignment and Spinning of Birefringent Particles Optically Trapped in an Array (AO)
• 3D manipulation of particles into crystal structures using holographic optical tweezers (OE)

Related Conference Papers

• Dynamically Adjustable Annular Laser Trapping for Sperm Chemotaxis Study
• Optical Trapping, Manipulation, and Sorting of Cells and Colloids in Microfluidic Systems with Diode Laser Bars
• Pulsed Optical Tweezers for Levitation and Manipulation of Stuck Biological Particles
• Interferometric Optical Sieve for Micro-Particle Segregation
• Observation of Orbital Angular Momentum Transfer to Particles Trapped in a Mathieu Beam