Two-photon bioimaging with picosecond optical pulses from a semiconductor laser

doi:10.1364/OE.14.003467

Two-photon bioimaging with picosecond optical pulses from a semiconductor laser

Hiroyuki Yokoyama, Hengchang Guo, Takuya Yoda, Keijiro Takashima, Ki-ichi Sato, Hirokazu Taniguchi, and Hiromasa Ito »View Author Affiliations

Optics Express, Vol. 14, Issue 8, pp. 3467-3471 (2006)
http://dx.doi.org/10.1364/OE.14.003467

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Abstract
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References (11)
Cited By
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Abstract

Toward a practical light source for two-photon bioimaging, we have generated kilowatt peak power of 0.77 μm wavelength and 5 ps optical pulse via second-harmonic generation of the amplified output from a gain-switched 1.55 μm semiconductor laser. This compact scheme and stable optical-pulse-source has been successfully used for the two-photon fluorescence bioimaging of actin filaments in PtK2 cells.

OCIS Codes
(140.5960) Lasers and laser optics : Semiconductor lasers
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Nonlinear Optics

History
Original Manuscript: February 17, 2006
Revised Manuscript: March 31, 2006
Manuscript Accepted: March 31, 2006
Published: April 17, 2006

Virtual Issues
Vol. 1, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Hiroyuki Yokoyama, Hengchang Guo, Takuya Yoda, Keijiro Takashima, Ki-ichi Sato, Hirokazu Taniguchi, and Hiromasa Ito, "Two-photon bioimaging with picosecond optical pulses from a semiconductor laser," Opt. Express 14, 3467-3471 (2006)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-14-8-3467

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References

W. Denk, J. H. Strickler, and W. W. Web, "Two-photon excitation in laser scanning microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]
K. König, "Multiphoton microscopy in life sciences," J. Microsc. 200,83-104 (2000). [CrossRef] [PubMed]
A. Zumbush, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999). [CrossRef]
P. F. Curley, A. I. Ferguson, J. G. White, and W. B. Amos, "Application of a femtosecond self-sustained mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy," Opt. Quantum Electron. 24,851-859 (1992). [CrossRef]
D. L. Wokosin, V. E. Centonze, S. Crittenden, and J. White, "Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser," Bioimaging 4,208-214 (1996). [CrossRef]
K. Svoboda, W. Denk, W. H. Knox, and S. Tsuda, "Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser," Opt. Lett. 21,1411-1413 (1996). [CrossRef] [PubMed]
J. Bewersdorf and S.W. Hell, "Picosecond pulsed two-photon imaging with repetition rates of 200 and 400 MHz," J. Microsc. 191,28-38 (1998). [CrossRef]
T. Yoda, H. Yokoyama, K. Sato, H. Taniguchi, and H. Ito, "High-peak-power picosecond optical-pulse generation with a gain-switched semiconductor laser, and high-efficiency wavelength conversion," presented at the Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR), CFM2-5, Tokyo, Japan, July 2005.
H. Yokoyama, M. Shirane, Y. Sasaki, H. Ito, and H. Taniguchi, "Supercontinuum generation in 800-nm wavelength region with semiconductor laser pulses," presented at the Nonlinear Optics, ThB3, Waikoloa, Hawaii, Aug. 2004.
Y. Kubota, T. Teshima, N. Nishimura, S. Kanto, S. Sakaguchi, Z. Meng, Y. Nakata, and T. Okada, "Novel Er and Ce codoped fluoride fiber amplifier for low-noise and high-efficient operation with 980-nm pumping," IEEE Photon. Technol. Lett. 15,525-527 (2003). [CrossRef]
H. Taniguchi, M. Kotoh, S. Maeda, K. Abe, and O. Tohyama, "Development of wavelength converter based on quasi-phase-matched PPMgLN waveguide," Mitsubishi Cable Ind. Rev.(JIHOU) 99,29-34 (2002).

Abe, K.
Amos, W. B.
Bewersdorf, J.
Centonze, V. E.
Crittenden, S.
Curley, P. F.
Denk, W.
Ferguson, A. I.
Hell, S.W.
Holtom, G. R.
Kanto, S.
Knox, W. H.
König, K.
Kotoh, M.
Kubota, Y.
Maeda, S.
Meng, Z.
Nakata, Y.
Nishimura, N.
Okada, T.
Sakaguchi, S.
Strickler, J. H.
Svoboda, K.
Taniguchi, H.
Teshima, T.
Tohyama, O.
Tsuda, S.
Web, W. W.
White, J.
White, J. G.
Wokosin, D. L.
Xie, X. S.
Zumbush, A.

Bioimaging

D. L. Wokosin, V. E. Centonze, S. Crittenden, and J. White, "Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser," Bioimaging 4,208-214 (1996). [CrossRef]

IEEE Photon. Technol. Lett.

Y. Kubota, T. Teshima, N. Nishimura, S. Kanto, S. Sakaguchi, Z. Meng, Y. Nakata, and T. Okada, "Novel Er and Ce codoped fluoride fiber amplifier for low-noise and high-efficient operation with 980-nm pumping," IEEE Photon. Technol. Lett. 15,525-527 (2003). [CrossRef]

J. Microsc.

J. Bewersdorf and S.W. Hell, "Picosecond pulsed two-photon imaging with repetition rates of 200 and 400 MHz," J. Microsc. 191,28-38 (1998). [CrossRef]
K. König, "Multiphoton microscopy in life sciences," J. Microsc. 200,83-104 (2000). [CrossRef] [PubMed]

Mitsubishi Cable Ind. Rev.

H. Taniguchi, M. Kotoh, S. Maeda, K. Abe, and O. Tohyama, "Development of wavelength converter based on quasi-phase-matched PPMgLN waveguide," Mitsubishi Cable Ind. Rev.(JIHOU) 99,29-34 (2002).

Opt. Lett.

K. Svoboda, W. Denk, W. H. Knox, and S. Tsuda, "Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser," Opt. Lett. 21,1411-1413 (1996). [CrossRef] [PubMed]

Opt. Quantum Electron.

P. F. Curley, A. I. Ferguson, J. G. White, and W. B. Amos, "Application of a femtosecond self-sustained mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy," Opt. Quantum Electron. 24,851-859 (1992). [CrossRef]

Phys. Rev. Lett.

A. Zumbush, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999). [CrossRef]

Science

W. Denk, J. H. Strickler, and W. W. Web, "Two-photon excitation in laser scanning microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]

Other

T. Yoda, H. Yokoyama, K. Sato, H. Taniguchi, and H. Ito, "High-peak-power picosecond optical-pulse generation with a gain-switched semiconductor laser, and high-efficiency wavelength conversion," presented at the Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR), CFM2-5, Tokyo, Japan, July 2005.
H. Yokoyama, M. Shirane, Y. Sasaki, H. Ito, and H. Taniguchi, "Supercontinuum generation in 800-nm wavelength region with semiconductor laser pulses," presented at the Nonlinear Optics, ThB3, Waikoloa, Hawaii, Aug. 2004.

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[1] W. Denk, J. H. Strickler, and W. W. Web, "Two-photon excitation in laser scanning microscopy," Science 248, 73-76 (1990). [CrossRef] [PubMed]

[2] K. König, "Multiphoton microscopy in life sciences," J. Microsc. 200,83-104 (2000). [CrossRef] [PubMed]

[3] A. Zumbush, G. R. Holtom, and X. S. Xie, "Three-dimensional vibrational imaging by coherent anti-Stokes Raman scattering," Phys. Rev. Lett. 82,4142-4145 (1999). [CrossRef]

[4] P. F. Curley, A. I. Ferguson, J. G. White, and W. B. Amos, "Application of a femtosecond self-sustained mode-locked Ti:sapphire laser to the field of laser scanning confocal microscopy," Opt. Quantum Electron. 24,851-859 (1992). [CrossRef]

[5] D. L. Wokosin, V. E. Centonze, S. Crittenden, and J. White, "Three-photon excitation fluorescence imaging of biological specimens using an all-solid-state laser," Bioimaging 4,208-214 (1996). [CrossRef]

[6] K. Svoboda, W. Denk, W. H. Knox, and S. Tsuda, "Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser," Opt. Lett. 21,1411-1413 (1996). [CrossRef] [PubMed]

[7] J. Bewersdorf and S.W. Hell, "Picosecond pulsed two-photon imaging with repetition rates of 200 and 400 MHz," J. Microsc. 191,28-38 (1998). [CrossRef]

[8] T. Yoda, H. Yokoyama, K. Sato, H. Taniguchi, and H. Ito, "High-peak-power picosecond optical-pulse generation with a gain-switched semiconductor laser, and high-efficiency wavelength conversion," presented at the Pacific Rim Conference on Lasers and Electro-Optics (CLEO-PR), CFM2-5, Tokyo, Japan, July 2005.

[9] H. Yokoyama, M. Shirane, Y. Sasaki, H. Ito, and H. Taniguchi, "Supercontinuum generation in 800-nm wavelength region with semiconductor laser pulses," presented at the Nonlinear Optics, ThB3, Waikoloa, Hawaii, Aug. 2004.

[10] Y. Kubota, T. Teshima, N. Nishimura, S. Kanto, S. Sakaguchi, Z. Meng, Y. Nakata, and T. Okada, "Novel Er and Ce codoped fluoride fiber amplifier for low-noise and high-efficient operation with 980-nm pumping," IEEE Photon. Technol. Lett. 15,525-527 (2003). [CrossRef]

[11] H. Taniguchi, M. Kotoh, S. Maeda, K. Abe, and O. Tohyama, "Development of wavelength converter based on quasi-phase-matched PPMgLN waveguide," Mitsubishi Cable Ind. Rev.(JIHOU) 99,29-34 (2002).

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Two-photon bioimaging with picosecond optical pulses from a semiconductor laser