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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

Quantitative imaging of complex samples by spiral phase contrast microscopy

Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, and Monika Ritsch-Marte  »View Author Affiliations

Optics Express, Vol. 14, Issue 9, pp. 3792-3805 (2006)

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Recently a spatial spiral phase filter in a Fourier plane of a microscopic imaging setup has been demonstrated to produce edge enhancement and relief-like shadow formation of amplitude and phase samples. Here we demonstrate that a sequence of at least 3 spatially filtered images, which are recorded with different rotational orientations of the spiral phase plate, can be used to obtain a quantitative reconstruction of both, amplitude and phase information of a complex microscopic sample, i.e. an object consisting of mixed absorptive and refractive components. The method is demonstrated using a calibrated phase sample, and an epithelial cheek cell.

© 2006 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(090.1970) Holography : Diffractive optics
(100.5090) Image processing : Phase-only filters

ToC Category:
Image Processing

Original Manuscript: March 8, 2006
Revised Manuscript: April 25, 2006
Manuscript Accepted: April 25, 2006
Published: May 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Stefan Bernet, Alexander Jesacher, Severin Fürhapter, Christian Maurer, and Monika Ritsch-Marte, "Quantitative imaging of complex samples by spiral phase contrast microscopy," Opt. Express 14, 3792-3805 (2006)

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