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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 1, Iss. 6 — Jun. 13, 2006

High-speed autofocusing of a cell using diffraction patterns

Hiromasa Oku, Masatoshi Ishikawa, Theodorus, and Koichi Hashimoto  »View Author Affiliations

Optics Express, Vol. 14, Issue 9, pp. 3952-3960 (2006)

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This paper proposes a new autofocusing method for observing cells under a transmission illumination. The focusing method uses a quick and simple focus estimation technique termed “depth from diffraction,” which is based on a diffraction pattern in a defocused image of a biological specimen. Since this method can estimate the focal position of the specimen from only a single defocused image, it can easily realize high-speed auto-focusing. To demonstrate the method, it was applied to continuous focus tracking of a swimming paramecium, in combination with two-dimensional position tracking. Three-dimensional tracking of the paramecium for 70 s was successfully demonstrated.

© 2006 Optical Society of America

OCIS Codes
(100.5010) Image processing : Pattern recognition
(170.5810) Medical optics and biotechnology : Scanning microscopy

ToC Category:

Original Manuscript: February 27, 2006
Revised Manuscript: April 12, 2006
Manuscript Accepted: April 13, 2006
Published: May 1, 2006

Virtual Issues
Vol. 1, Iss. 6 Virtual Journal for Biomedical Optics

Hiromasa Oku, Masatoshi Ishikawa, Theodorus , and Koichi Hashimoto, "High-speed autofocusing of a cell using diffraction pattern," Opt. Express 14, 3952-3960 (2006)

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