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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 7 — Jul. 16, 2007

Fluctuations in closed-loop fluorescent particle tracking

Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi  »View Author Affiliations


Optics Express, Vol. 15, Issue 12, pp. 7752-7773 (2007)
http://dx.doi.org/10.1364/OE.15.007752


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Abstract

We present a comprehensive theory of closed-loop particle tracking for calculating the statistics of a diffusing fluorescent particle’s motion relative to the tracking lock point. A detailed comparison is made between the theory and experimental results, with excellent quantitative agreement found in all cases. A generalization of the theory of (open-loop) fluorescence correlation spectroscopy is developed, and the relationship to previous results is discussed. Two applications of the statistical techniques are given: a method for determining a tracked particle’s localization and an algorithm for rapid particle classification based on real-time analysis of the tracking control signal.

© 2007 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: May 4, 2007
Revised Manuscript: June 6, 2007
Manuscript Accepted: June 6, 2007
Published: June 7, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Andrew J. Berglund, Kevin McHale, and Hideo Mabuchi, "Fluctuations in closed-loop fluorescent particle tracking," Opt. Express 15, 7752-7773 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-12-7752


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