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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 7 — Jul. 16, 2007

Fully automated beam-alignment and single stroke guided manual alignment of counter-propagating multi-beam based optical micromanipulation systems

Jeppe Seidelin Dam, Peter John Rodrigo, Ivan R. Perch-Nielsen, and Jesper Glückstad  »View Author Affiliations

Optics Express, Vol. 15, Issue 13, pp. 7968-7973 (2007)

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In a previous paper [J. S. Dam et al, Opt. Express 15, 1923 (2007)] we demonstrated computerized “drag-and-drop” optical alignment of a counter-propagating multi-beam based micromanipulation system. By inclusion of image analysis, we report here on the extension of this work to accommodate a completely automated beam-alignment process. Additionally, to maintain a cost-effective and technically less demanding system architecture, we also report on a computer-guided manual alignment procedure. In the manual version, the computer analyzes the initial misalignment and the required compensations for each mirror in the system are calculated. Subsequently, the user is guided in adjusting the mirrors exactly by the requisite amount. This way, all mirrors only need to be moved once. The image analysis utilized in both calibration schemes employs a fitting algorithm to determine the position of beam-center with sub-pixel accuracy, thereby providing “better than human” alignment.

© 2007 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(170.4520) Medical optics and biotechnology : Optical confinement and manipulation
(220.1140) Optical design and fabrication : Alignment

ToC Category:

Original Manuscript: May 3, 2007
Revised Manuscript: June 6, 2007
Manuscript Accepted: June 7, 2007
Published: June 11, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Jeppe Seidelin Dam, Peter John Rodrigo, Ivan R. Perch-Nielsen, and Jesper Glückstad, "Fully automated beam-alignment and single stroke guided manual alignment of counter-propagating multi-beam based optical micromanipulation systems," Opt. Express 15, 7968-7973 (2007)

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