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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans

Frederic Bourgeois and Adela Ben-Yakar  »View Author Affiliations

Optics Express, Vol. 15, Issue 14, pp. 8521-8531 (2007)

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We present a study characterizing the properties of femtosecond laser nanosurgery applied to individual axons in live Caenorhabditis elegans (C. elegans) using nano-Joule laser pulses at 1 kHz repetition rate. Emphasis is placed on the characterization of the damage threshold, the extent of damage, and the statistical rates of axonal recovery as a function of laser parameters. The ablation threshold decreases with increasing number of pulses applied during nanoaxotomy. This dependency suggests the existence of an incubation effect. In terms of extent of damage, the energy per pulse is found to be a more critical parameter than the number of pulses. Axonal recovery improves when surgery is performed using a large number of low energy pulses.

© 2007 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.1020) Medical optics and biotechnology : Ablation of tissue
(190.4180) Nonlinear optics : Multiphoton processes

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: March 8, 2007
Revised Manuscript: May 14, 2007
Manuscript Accepted: June 5, 2007
Published: June 25, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Frederic Bourgeois and Adela Ben-Yakar, "Femtosecond laser nanoaxotomy properties and their effect on axonal recovery in C. elegans," Opt. Express 15, 8521-8531 (2007)

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