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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Development of fluorescent materials for Diffuse Fluorescence Tomography standards and phantoms

John Baeten, Mark Niedre, Joshua Dunham, and Vasilis Ntziachristos  »View Author Affiliations


Optics Express, Vol. 15, Issue 14, pp. 8681-8694 (2007)
http://dx.doi.org/10.1364/OE.15.008681


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Abstract

The availability of fluorescence standards is necessary in the development of systems and methods for fluorescence imaging. In this study, two approaches for developing diffuse fluorescence materials to be used as standards or phantoms in diffuse fluorescent tomography applications were investigated. Specifically, silicone rubber and polyester casting resin were used as base materials, and silicone pigments or TiO2/ India Ink were added respectively to vary the optical properties. Characterization of the optical properties achieved was performed using time-resolved methods. Subsequently, different near-infrared fluorochromes were examined for imparting controlled and stable fluorescence properties. It was determined that hydrophobic fluorophores (IR 676 and IR 780 Iodide) suspended in dichloromethane and hydrophilic fluorophores (Cy5.5 and AF 750) suspended in methanol produced diffusive silicone and resin fluorescent materials, respectively. However only the hydrophobic fluorophores embedded within silicone resulted in the construction of a material with the characteristics of a standard, i.e. stability of fluorescence intensity with time and a linear dependence of normalized fluorescence intensity to fluorophore concentration.

© 2007 Optical Society of America

OCIS Codes
(120.4800) Instrumentation, measurement, and metrology : Optical standards and testing
(160.2540) Materials : Fluorescent and luminescent materials
(160.4760) Materials : Optical properties

ToC Category:
Instrumentation, Measurement, and Metrology

History
Original Manuscript: November 21, 2006
Revised Manuscript: January 14, 2007
Manuscript Accepted: February 2, 2007
Published: June 27, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

Citation
John Baeten, Mark Niedre, Joshua Dunham, and Vasilis Ntziachristos, "Development of fluorescent materials for Diffuse Fluorescence Tomography standards and phantoms," Opt. Express 15, 8681-8694 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-14-8681


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References

  1. H. R. Herschman, "Molecular imaging: looking at problems, seeing solutions," Science 302, 605-8 (2003). [CrossRef] [PubMed]
  2. V. Ntziachristos, J. Ripoll, L. H. V. Wang, and R. Weissleder, "Looking and listening to light: the evolution of whole-body photonic imaging," Nat. Biotechnol. 23, 313-320 (2005). [CrossRef] [PubMed]
  3. M. Gurfinkel, S. Ke, X. X. Wen, C. Li, and E. M. Sevick-Muraca, "Near-infrared fluorescence optical imaging and tomography," Disease Markers 19, 107-121 (2003).
  4. M. J. Eppstein, D. J. Hawrysz, A. Godavarty, and E. M. Sevick-Muraca, "Three-dimensional, Bayesian image reconstruction from sparse and noisy data sets: Near-infrared fluorescence tomography," Proc. Natl Acad. Sci. USA 99, 9619-9624 (2002). [CrossRef] [PubMed]
  5. J. H. Chang, H. L. Graber, and R. L. Barbour, "Imaging of fluorescence in highly scattering media," IEEE Trans. Biomed. Eng. 44, 810-822 (1997). [CrossRef] [PubMed]
  6. H. B. Jiang, S. Ramesh, and M. Bartlett, "Combined optical and fluorescence imaging for breast cancer detection and diagnosis," Crit. Rev. Biomed. Eng. 28, 371-375 (2000). [PubMed]
  7. S. V. Patwardhan, S. R. Bloch, S. Achilefu, and J. P. Culver, "Time-dependent whole-body fluorescence tomography of probe bio-distributions in mice," Opt. Express 13, 2564-2577 (2005). [CrossRef] [PubMed]
  8. R. Weissleder and V. Ntziachristos, "Shedding light onto live molecular targets," Nat. Med. 9, 123-128 (2003). [CrossRef] [PubMed]
  9. A. D. Klose and A. H. Hielscher, "Fluorescence tomography with simulated data based on the equation of radiative transfer," Opt. Lett. 28, 1019-1021 (2003). [CrossRef] [PubMed]
  10. A. D. Klose, V. Ntziachristos, and A. H. Hielscher, "The inverse source problem based on the radiative transfer equation in optical molecular imaging," J. Comput. Phys. 202, 323-345 (2005). [CrossRef]
  11. U. Resch-Genger, K. Hoffmann, W. Nietfeld, A. Engel, J. Neukammer, R. Nitschke, B. Ebert, and R. Macdonald, "How to improve quality assurance in Fluorometry: fluorescence-inherent sources of error and suited fluorescence standards," J. Fluoresc. 15, 337-362 (2005). [CrossRef] [PubMed]
  12. M. Firbank and D. Delpy, "A design for a stable and reproducible phantom for use in near-infrared imaging and spectroscopy," Phys. Med. Biol. 38, 847-853 (1993). [CrossRef]
  13. T. Moffitt, Y. Chen, and S. Prahl, "Preparation and characterization of polyurethane optical phantoms," J. Biomed. Opt. 11, 041103 (2006). [CrossRef] [PubMed]
  14. M. Niedre, G. Turner, and V. Ntziachristos, "Time-resolved imaging of optical coefficients through murine chest cavities," J. Biomed. Opt.in press (2006). [CrossRef]
  15. M. S. Patterson, B. Chance, and B. C. Wilson, "Time resolved reflectance and transmittance for the noninvasive measurement of tissue optical-properties," Appl. Opt. 28, 2331-2336 (1989). [CrossRef] [PubMed]
  16. E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, "A sub-millimeter resolution fluorescence molecular imaging system for small animal imaging," Med. Phys. 30, 901-911 (2003). [CrossRef] [PubMed]
  17. V. Ntziachristos and R. Weissleder, "Experimental three-dimensional fluorescence reconstruction of diffuse media using a normalized born approximation," Opt. Lett. 26, 893-895 (2001). [CrossRef]
  18. A. Soubret, J. Ripoll, and V. Ntziachristos, "Accuracy of fluorescent tomography in the presence of heterogeneities: Study of the normalized Born ratio." IEEE Trans. Med. Imaging 24, 1377-1386 (2005). [CrossRef] [PubMed]

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