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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 8 — Aug. 10, 2007

Development of fluorescent materials for Diffuse Fluorescence Tomography standards and phantoms

John Baeten, Mark Niedre, Joshua Dunham, and Vasilis Ntziachristos  »View Author Affiliations

Optics Express, Vol. 15, Issue 14, pp. 8681-8694 (2007)

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The availability of fluorescence standards is necessary in the development of systems and methods for fluorescence imaging. In this study, two approaches for developing diffuse fluorescence materials to be used as standards or phantoms in diffuse fluorescent tomography applications were investigated. Specifically, silicone rubber and polyester casting resin were used as base materials, and silicone pigments or TiO2/ India Ink were added respectively to vary the optical properties. Characterization of the optical properties achieved was performed using time-resolved methods. Subsequently, different near-infrared fluorochromes were examined for imparting controlled and stable fluorescence properties. It was determined that hydrophobic fluorophores (IR 676 and IR 780 Iodide) suspended in dichloromethane and hydrophilic fluorophores (Cy5.5 and AF 750) suspended in methanol produced diffusive silicone and resin fluorescent materials, respectively. However only the hydrophobic fluorophores embedded within silicone resulted in the construction of a material with the characteristics of a standard, i.e. stability of fluorescence intensity with time and a linear dependence of normalized fluorescence intensity to fluorophore concentration.

© 2007 Optical Society of America

OCIS Codes
(120.4800) Instrumentation, measurement, and metrology : Optical standards and testing
(160.2540) Materials : Fluorescent and luminescent materials
(160.4760) Materials : Optical properties

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: November 21, 2006
Revised Manuscript: January 14, 2007
Manuscript Accepted: February 2, 2007
Published: June 27, 2007

Virtual Issues
Vol. 2, Iss. 8 Virtual Journal for Biomedical Optics

John Baeten, Mark Niedre, Joshua Dunham, and Vasilis Ntziachristos, "Development of fluorescent materials for Diffuse Fluorescence Tomography standards and phantoms," Opt. Express 15, 8681-8694 (2007)

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