Whole spectrum fluorescence detection with ultrafast white light excitation
Optics Express, Vol. 15, Issue 16, pp. 10439-10445 (2007)
http://dx.doi.org/10.1364/OE.15.010439
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Abstract
We have developed a new detection mechanism for ultrabroadband multicolor fluorescence detection using an ultrafast supercontinuum white light source without spectral filtering to simultaneously excite different fluorophores. A nonlinear photonic crystal fiber was utilized in conjunction with a femtosecond laser to generate the supercontinuum. A time-resolved detector was tested to detect the whole spectrum fluorescence while gating out the excitation white light in the time domain.
© 2007 Optical Society of America
OCIS Codes
(120.4640) Instrumentation, measurement, and metrology : Optical instruments
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.7160) Medical optics and biotechnology : Ultrafast technology
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6500) Spectroscopy : Spectroscopy, time-resolved
ToC Category:
Spectroscopy
History
Original Manuscript: June 12, 2007
Revised Manuscript: July 24, 2007
Manuscript Accepted: July 25, 2007
Published: August 2, 2007
Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Jing Yong Ye, Charles J. Divin, James R. Baker, and Theodore B. Norris, "Whole spectrum fluorescence detection with ultrafast white light excitation," Opt. Express 15, 10439-10445 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-16-10439
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