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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Whole spectrum fluorescence detection with ultrafast white light excitation

Jing Yong Ye, Charles J. Divin, James R. Baker, Jr., and Theodore B. Norris  »View Author Affiliations


Optics Express, Vol. 15, Issue 16, pp. 10439-10445 (2007)
http://dx.doi.org/10.1364/OE.15.010439


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Abstract

We have developed a new detection mechanism for ultrabroadband multicolor fluorescence detection using an ultrafast supercontinuum white light source without spectral filtering to simultaneously excite different fluorophores. A nonlinear photonic crystal fiber was utilized in conjunction with a femtosecond laser to generate the supercontinuum. A time-resolved detector was tested to detect the whole spectrum fluorescence while gating out the excitation white light in the time domain.

© 2007 Optical Society of America

OCIS Codes
(120.4640) Instrumentation, measurement, and metrology : Optical instruments
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.7160) Medical optics and biotechnology : Ultrafast technology
(300.2530) Spectroscopy : Fluorescence, laser-induced
(300.6500) Spectroscopy : Spectroscopy, time-resolved

ToC Category:
Spectroscopy

History
Original Manuscript: June 12, 2007
Revised Manuscript: July 24, 2007
Manuscript Accepted: July 25, 2007
Published: August 2, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Jing Yong Ye, Charles J. Divin, James R. Baker, and Theodore B. Norris, "Whole spectrum fluorescence detection with ultrafast white light excitation," Opt. Express 15, 10439-10445 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-16-10439


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References

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