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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 9 — Sep. 26, 2007

Phosphorescence lifetime based oxygen micro-sensing using a digital micromirror device

Shih-hui Chao, Mark R. Holl, Sarah C. McQuaide, Timothy T. H. Ren, Steven A. Gales, and Deirdre R. Meldrum  »View Author Affiliations


Optics Express, Vol. 15, Issue 17, pp. 10681-10689 (2007)
http://dx.doi.org/10.1364/OE.15.010681


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Abstract

A digital light modulation microscope (DLMM) that utilizes a digital micromirror device (DMD) on an epifluorescence microscope has been developed to modulate excitation light in spatial and temporal domains for phosphorescence lifetime detection. Local O2 concentration can be inferred through the detected lifetime around an O2-quenching phosphorescent porphyrin microsensor. Combined with microsensor arrays, the DLMM can sequentially address light to each microsensor element to construct a discrete lifetime image or O2 distribution. In contrast to conventional phosphorescence lifetime imaging, the new method eliminates the need for a pulsed light source and a time-gated camera. To demonstrate O2 sensing with lab-on-a-chip devices, an array of 150-µm-diameter micro-wells coated with phosphorescent porphyrin were observed. The locations of the sensor elements were automatically identified though image analysis. The goal of this platform is to measure the O2 consumption of individual cells trapped in the microwells.

© 2007 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(230.2090) Optical devices : Electro-optical devices
(230.3990) Optical devices : Micro-optical devices
(230.6120) Optical devices : Spatial light modulators

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 11, 2007
Revised Manuscript: July 29, 2007
Manuscript Accepted: July 31, 2007
Published: August 8, 2007

Virtual Issues
Vol. 2, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Shih-hui Chao, Mark R. Holl, Sarah C. McQuaide, Timothy T. H. Ren, Steven A. Gales, and Deirdre R. Meldrum, "Phosphorescence lifetime based oxygen micro-sensing using a digital micromirror device," Opt. Express 15, 10681-10689 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-17-10681


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