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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 11 — Nov. 26, 2007

Luminescent images of single gold nanoparticles and their labeling on silica beads

Jian Zhang, Yi Fu, and Joseph R. Lakowicz  »View Author Affiliations


Optics Express, Vol. 15, Issue 20, pp. 13415-13420 (2007)
http://dx.doi.org/10.1364/OE.15.013415


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Abstract

Luminescent Au nanoparticles were synthesized in a modified Brust method (average diameters of metal core = 1.6 nm). The fluorescence images were measured using scanning confocal microscopy and validated as compared with organic fluorophores. The metal particles were functionalized with succinimidyl ester terminated ligands and bound as fluorophores on surface-aminated silica beads to mimic labeling of biological functionalities. The labeled silica beads were shown to display bright signals and good photostability. Our results indicate that the luminescent metal nanoparticles can be employed as the probes to label the biological functionalities in developing molecule imaging agents.

© 2007 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optics at Surfaces

History
Original Manuscript: August 3, 2007
Revised Manuscript: September 25, 2007
Manuscript Accepted: September 26, 2007
Published: September 28, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Jian Zhang, Yi Fu, and Joseph R. Lakowicz, "Luminescent images of single gold nanoparticles and their labeling on silica beads," Opt. Express 15, 13415-13420 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-20-13415


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References

  1. R. Weissleder, C. H. Tung, U. Mahmood, and A. BogdanovJr., "A in vivo imaging of tumors with protease-activated near-infrared fluorescent probes," Nature Biotechnol. 17, 375-378 (1999). [CrossRef]
  2. D. J. Hawrysz and E. M. Sevick-Muraca, "Developments toward diagnostic breast cancer imaging using near-infrared optical measurements and fluorescent contrast agents," Neoplasia 2, 388-417(2000). [CrossRef]
  3. J.-P. Knemeyer, D.-P. Herten, and M. Sauer, "Detection and identification of single molecules in living cells using spectrally resolved fluorescence lifetime imaging microscopy," Anal. Chem. 75, 2147-2153 (2003). [CrossRef] [PubMed]
  4. J. Nakanishi, T. Nakajima, M. Sato, T. Ozawa, K. Tohda, and Y. Umezawa, "Imaging of conformational changes of proteins with a new environment-sensitive fluorescent probe designed for site-specific labeling of recombinant proteins in live cells," Anal. Chem. 73, 2920-2928 (2001). [CrossRef] [PubMed]
  5. V. Ntziachristos and B. Chance, "Breast imaging technology: Probing physiology and molecular function using optical imaging - applications to breast cancer," Breast Cancer Res. 3, 41-46 (2001). [CrossRef] [PubMed]
  6. P. V. Kamat, "Photophysical, photochemical and photocatalytic aspects of metal nanoparticles," J. Phys. Chem. B 106, 7729 (2002). [CrossRef]
  7. M.-C. Daniel and D. Astruc, "Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology," Chem. Rev. 104, 293-346 (2004). [CrossRef] [PubMed]
  8. N. L. Rosi, and C. A. Mirkin, "Nanostructures in biodiagnostics," Chem. Rev. 105, 1547-1562 (2005). [CrossRef] [PubMed]
  9. R. M. Penner, "Hybrid electrochemical/chemical synthesis of quantum dots," Acc. Chem. Res. 33, 78-86 (2003). [CrossRef]
  10. G. Markovich, C. P. Collier, S. E. Henrichs, F. Remacle, R. D. Levine, and J. R. Heath, "Architectonic quantum dot solids," Acc. Chem. Res. 32, 415-423 (1999). [CrossRef]
  11. C. Landes, M. Braun, C. Burda, and M. A. El-Sayed, "Observation of large changes in the band gap absorption energy of small CdSe nanoparticles induced by the adsorption of a strong hole acceptor" Nano Lett. 1, 667-670 (2001). [CrossRef]
  12. M. A. El-Sayed, "Small is different: shape-, size-, and composition-dependent properties of some colloidal semiconductor nanocrystals," Acc. Chem. Res. 37, 326-333 (2004). [CrossRef] [PubMed]
  13. J. R. Reimers and N. S. Hush, "The need for quantum-mechanical treatment of capacitance and related properties of nanoelectrodes," J. Phys. Chem. B 105, 8979-8988 (2001). [CrossRef]
  14. G. Wang, R. Guo, G. Kalyuzhny, J.-P. Choi, and R. W. Murray, "NIR luminescence intensities increase linearly with proportion of polar thiolate ligands in protecting monolayers of Au38 and Au140 quantum dots," J. Phys. Chem. B. 110, 20282-20289 (2006). [CrossRef] [PubMed]
  15. J. Zheng, C. Zhang, and R. M. Dickson, "Highly fluorescent, water-soluble, size-tunable gold quantum dots," Phys. Rev. Lett. 93, 077402 (2004). [CrossRef] [PubMed]
  16. T. Huang, and R. W. Murray, "Visible luminescence of water-soluble monolayer-protected gold clusters," J. Phys. Chem. B 105, 12498-12502 (2001). [CrossRef]
  17. T. P. Bigioni, R. L. Whetten, and O. Dag, "Near-infrared luminescence from small gold nanocrystals," J. Phys. Chem. B 104, 6983-6986 (2000). [CrossRef]
  18. S. Link, A. Beeby, S. FitzGerald, M. A. El-Sayed, T. G. Schaaff, and R. L. Whetten, "Visible to infrared luminescence from a 28-Atom gold cluster," J. Phys. Chem. B 106, 3410-3415 (2002). [CrossRef]
  19. Q.-M. Wang, Y.-A. Lee, O. Crespo, J. Deaton, C. Tang, H. J. Gysling, G. M. Concepcion, C. Larraz, M. D. Villacampa, A. Laguna, and R. Eisenberg, "Intensely luminescent gold(I)-silver(I) cluster complexes with tunable structural features, J. Am. Chem. Soc. 126, 9488-9489 (2004). [CrossRef] [PubMed]
  20. G. Wang, T. Huang, R. W. Murray, L. Menard, and R. G. Nuzzo, "Near-IR luminescence of monolayer-protected metal clusters," J. Am. Chem. Soc. 127, 812-813 (2005). [CrossRef] [PubMed]
  21. J. Zhang, D. Roll, C. D. Geddes, and J. R. Lakowicz, "Aggregation of silver nanoparticle-dextran adducts with concanavalin A and competitive complexation with glucose," J. Phys. Chem. B 108, 12210-12214 (2004). [CrossRef]
  22. J. Zhang and J. R. Lakowicz, "Enhanced luminescence of phenyl-phenanthridine dye on aggregated small silver nanoparticles," J. Phys. Chem. B. 109, 8701-8706 (2005). [CrossRef]
  23. R. S. Ingram, M. J. Hostetler, and R. W. Murray, "Poly-hetero--functionalized alkanethiolate-stabilized gold cluster compounds," J. Am. Chem. Soc. 119, 9175-9178 (1997). [CrossRef]
  24. W. Stöber, A. Fink, E. Bohn, "A novel method for synthesis of silica nanoparticles," J. Colloid Interface Sci. 26, 62-68 (1968).
  25. J. Zhang, Y. Fu, and J. R. Lakowicz, "Dye-labeled silver nanoshell-bright particle," J. Phys. Chem. B. 110, 8986-8991 (2006). [CrossRef] [PubMed]
  26. J. Zhang, Y. Fu, M. H. Chowdhury, and J. R. Lakowicz, "Metal-enhanced single-molecule fluorescence on silver particle monomer and dimer: coupling effect between metal particles," Nano Lett. 7, 2101-2107 (2007). [CrossRef] [PubMed]
  27. T. Vosch, Y. Antoku, J.-C. Hsiang, C. I. Richards, J. I. Gonzalez, and R. M. Dickson, "Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores," PNAS 104, 12616-12621 (2007). [CrossRef] [PubMed]

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