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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 11 — Nov. 26, 2007

Luminescent images of single gold nanoparticles and their labeling on silica beads

Jian Zhang, Yi Fu, and Joseph R. Lakowicz  »View Author Affiliations

Optics Express, Vol. 15, Issue 20, pp. 13415-13420 (2007)

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Luminescent Au nanoparticles were synthesized in a modified Brust method (average diameters of metal core = 1.6 nm). The fluorescence images were measured using scanning confocal microscopy and validated as compared with organic fluorophores. The metal particles were functionalized with succinimidyl ester terminated ligands and bound as fluorophores on surface-aminated silica beads to mimic labeling of biological functionalities. The labeled silica beads were shown to display bright signals and good photostability. Our results indicate that the luminescent metal nanoparticles can be employed as the probes to label the biological functionalities in developing molecule imaging agents.

© 2007 Optical Society of America

OCIS Codes
(010.1080) Atmospheric and oceanic optics : Active or adaptive optics
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Optics at Surfaces

Original Manuscript: August 3, 2007
Revised Manuscript: September 25, 2007
Manuscript Accepted: September 26, 2007
Published: September 28, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Jian Zhang, Yi Fu, and Joseph R. Lakowicz, "Luminescent images of single gold nanoparticles and their labeling on silica beads," Opt. Express 15, 13415-13420 (2007)

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