OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 2, Iss. 11 — Nov. 26, 2007

Photon statistics in single molecule orientational imaging

Matthew R. Foreman, Sherif S. Sherif, and Peter Türük  »View Author Affiliations

Optics Express, Vol. 15, Issue 21, pp. 13597-13606 (2007)

View Full Text Article

Enhanced HTML    Acrobat PDF (242 KB) Open Access

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Optical techniques in single molecule imaging rely heavily on photon counting for data acquisition. Extraction of information from the recorded readings is often done by means of statistical signal processing, however this requires a full knowledge of the photoelectron statistics. In addition to counting statistics we include a specific form of random signal variations namely reorientational dynamics, or wobble to derive the general probability density function of the number of detected photons. The relative importance of the two factors is dependent upon the total number of photons in the system and results are given in all regimes.

© 2007 Optical Society of America

OCIS Codes
(000.5490) General : Probability theory, stochastic processes, and statistics
(110.4280) Imaging systems : Noise in imaging systems
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Imaging Systems

Original Manuscript: May 17, 2007
Revised Manuscript: September 28, 2007
Manuscript Accepted: September 28, 2007
Published: October 2, 2007

Virtual Issues
Vol. 2, Iss. 11 Virtual Journal for Biomedical Optics

Matthew R. Foreman, Sherif S. Sherif, and Peter Török, "Photon statistics in single molecule orientational imaging," Opt. Express 15, 13597-13606 (2007)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. K. D. Weston and L. S. Goldner "Orientation imaging and reorientation dynamics of single dye molecules," J. Phys. Chem. B 105, 3453-3462 (2001). [CrossRef]
  2. D. M. Warshaw, E. Hayes, D. Gaffney, A. M. Lauzon, J. R. Wu, G. Kennedy, K. Trybus, S. Lowey, and C. Berger, "Myosin conformational states determined by single fluorophore polarization," Proc. Natl. Acad. Sci. U.S.A. 95, 8034-8039 (1998). [CrossRef] [PubMed]
  3. H. P. Lu, L. Y. Xun, and X. S. Xie, "Single molecule enzymatic dynamics," Science 282, 1877-1882 (1998). [CrossRef] [PubMed]
  4. R. E. Dale and S. C. Hopkins "Model-Independent analysis of the orientation of fluorescent probes with restricted mobility in muscle fibers," Biophys. J. 76, 1606-1618 (1999). [CrossRef] [PubMed]
  5. T. M. Jovin, M. Bartholdi, W. L. C. Vaz, and R. H. Austin, "Rotational diffusion of biological macromolecules by time-resolved delayed luminescence (phosphorescence, fluorescence) anisotropy," Ann. N. Y. Acad. Sci. 366, 176-196 (1981). [CrossRef] [PubMed]
  6. T. Ha, T. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin and S. Weiss, "Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor," Proc. Natl. Acad. Sci. U.S.A. 93, 6264-6268 (1996). [CrossRef] [PubMed]
  7. T. Ha, J. Glass, T. Enderle, D. S. Chemla, and S. Weiss, "Hindered rotational diffusion and rotational jumps of single molecules," Phys. Rev. Lett. 80, 2093-2096 (1998). [CrossRef]
  8. Th. Basché, W. P. Ambrose, and W. E. Moerner, "Optical spectra and kinetics of single impurity molecules in a polymer: spectral diffusion and persistent spectral hole burning," J. Opt. Soc. Am. B. 9, 829-836 (1992). [CrossRef]
  9. G. H. Patterson, S. N. Knobel, W. D. Sharif, S. R. Kain, and D. W. Piston, "Use of the green flurorescent protein and its mutants in quantitative fluorescence microscopy," Biophys. J. 73, 2782-2790 (1997). [CrossRef] [PubMed]
  10. D. J. Pikas, S. M. Kirkpatrick, E. Tewksbury, L. L. Brott, R. R. Naik, M. O. Stone, and W.M. Dennis, "Nonlinear saturation and lasing characteristics of green fluorescent protein," J. Phys. Chem. B 106, 4831-4837 (2002). [CrossRef]
  11. A. Leon-Garcia, Probability and Random Processes for Electrical Engineering (Addison-Wesley Publishing Company Inc., 1994).
  12. T. Ha, T. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, "Single molecule dynamics studied by polarization modulation," Phys. Rev. Lett. 77, 3979-3982 (1996). [CrossRef] [PubMed]
  13. B. Sick, B. Hecht, and L. Novotny "Orientational imaging of single molecules by annular illumination," Phys. Rev. Lett. 85, 4482-4485 (2000). [CrossRef] [PubMed]
  14. R. M. Dickson, D. J. Norris, and W. E. Moerner, "Simultaneous imaging of individual molecules aligned both parallel and perpendicular to the optic axis," Phys. Rev. Lett. 81, 5322-5325 (1998). [CrossRef]
  15. K. Itô, Introduction to Probability Theory (Cambridge University Press, 1984).
  16. I. S. Gradshteyn and I. M. Ryzhik, Table of Integrals, Series and Products (Academic Press, London, 1980).
  17. W. Feller, Probability Theory and its Applications (John Wiley and Sons Inc., New York, 1950).
  18. P. Debye, Polar Molecules (Dover Publications, New York, 1945).
  19. P. Wahl, K. Tawada, and J. C. Auchet, "Study of tropomyosin labelled with a fluorescent probe by pulse fluorimetry in polarized light. Interaction of that protein with troponin and actin," Eur. J. Biochem. 88, 421-424 (1978). [CrossRef] [PubMed]
  20. J. Yguerabide, H. F. Epstein, and L. Stryer, "Segmental flexibility in an antibody molecule," J. Mol. Biol. 51, 573-590 (1970). [CrossRef] [PubMed]
  21. W. E. Moerner and D. P. Fromm, "Methods of single molecule fluorescence spectroscopy and microscopy," Rev. Sci. Instrum. 74, 3597-3619 (2003). [CrossRef]
  22. I. Munro, I. Pecht, and L. Stryer "Subnanosecond motions of Tryptophan residues in proteins," Proc. Natl. Acad. Sci. USA 76, 56-60 (1979). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited