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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples

Jigang Wu, Zahid Yaqoob, Xin Heng, Xiquan Cui, and Changhuei Yang  »View Author Affiliations

Optics Express, Vol. 15, Issue 26, pp. 18141-18155 (2007)

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We have developed a full-field high resolution quantitative phase imaging technique for observing dynamics of transparent biological samples. By using a harmonically matched diffraction grating pair (600 and 1200 lines/mm), we were able to obtain non-trivial phase difference (other than 0° or 180°) between the output ports of the gratings. Improving upon our previous design, our current system mitigates astigmatism artifacts and is capable of high resolution imaging. This system also employs an improved phase extraction algorithm. The system has a lateral resolution of 1.6 µm and a phase sensitivity of 62 mrad. We employed the system to acquire high resolution phase images of onion skin cells and a phase movie of amoeba proteus in motion.

© 2007 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.3880) Medical optics and biotechnology : Medical and biological imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 6, 2007
Revised Manuscript: December 4, 2007
Manuscript Accepted: December 14, 2007
Published: December 19, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Jigang Wu, Zahid Yaqoob, Xin Heng, Xiquan Cui, and Changhuei Yang, "Harmonically matched grating-based full-field quantitative high-resolution phase microscope for observing dynamics of transparent biological samples," Opt. Express 15, 18141-18155 (2007)

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