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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Adaptive optics for enhanced signal in CARS microscopy

A. J. Wright, S. P. Poland, J. M. Girkin, C. W. Freudiger, C. L. Evans, and X. S. Xie  »View Author Affiliations


Optics Express, Vol. 15, Issue 26, pp. 18209-18219 (2007)
http://dx.doi.org/10.1364/OE.15.018209


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Abstract

We report the use of adaptive optics with coherent anti-Stokes Raman scattering (CARS) microscopy for label-free deep tissue imaging based on molecular vibrational spectroscopy. The setup employs a deformable membrane mirror and a random search optimization algorithm to improve signal intensity and image quality at large sample depths. We demonstrate the ability to correct for both system and sample-induced aberrations in test samples as well as in muscle tissue in order to enhance the CARS signal. The combined system and sample-induced aberration correction increased the signal by an average factor of ~3x for the test samples at a depth of 700 µm and ~6x for muscle tissue at a depth of 260 µm. The enhanced signal and higher penetration depth offered by adaptive optics will augment CARS microscopy as an in vivo and in situ biomedical imaging modality.

© 2007 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(220.1000) Optical design and fabrication : Aberration compensation
(300.6230) Spectroscopy : Spectroscopy, coherent anti-Stokes Raman scattering
(180.4315) Microscopy : Nonlinear microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: October 30, 2007
Revised Manuscript: December 14, 2007
Manuscript Accepted: December 17, 2007
Published: December 19, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Citation
A. J. Wright, S. P. Poland, J. M. Girkin, C. W. Freudiger, C. L. Evans, and X. S. Xie, "Adaptive optics for enhanced signal in CARS microscopy," Opt. Express 15, 18209-18219 (2007)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-15-26-18209


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