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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 1 — Jan. 29, 2008

Characterizing and tracking single colloidal particles with video holographic microscopy

Sang-Hyuk Lee, Yohai Roichman, Gi-Ra Yi, Shin-Hyun Kim, Seung-Man Yang, Alfons van Blaaderen, Peter van Oostrum, and David G. Grier  »View Author Affiliations

Optics Express, Vol. 15, Issue 26, pp. 18275-18282 (2007)

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We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere’s radius and refractive index to within 1%, and simultaneously to measure its three-dimensional position with nanometer in-plane precision and 10 nanometer axial resolution.

© 2007 Optical Society of America

OCIS Codes
(090.1760) Holography : Computer holography
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:

Original Manuscript: October 12, 2007
Revised Manuscript: December 9, 2007
Manuscript Accepted: December 17, 2007
Published: December 20, 2007

Virtual Issues
Vol. 3, Iss. 1 Virtual Journal for Biomedical Optics

Sang-Hyuk Lee, Yohai Roichman, Gi-Ra Yi, Shin-Hyun Kim, Seung-Man Yang, Alfons van Blaaderen, Peter van Oostrum, and David G. Grier, "Characterizing and tracking single colloidal particles with video holographic microscopy," Opt. Express 15, 18275-18282 (2007)

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