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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer

Adam E. Cohen and W. E. Moerner  »View Author Affiliations


Optics Express, Vol. 16, Issue 10, pp. 6941-6956 (2008)
http://dx.doi.org/10.1364/OE.16.006941


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Abstract

We present an Anti-Brownian Electrokinetic trap (ABEL trap) capable of trapping individual fluorescently labeled protein molecules in aqueous buffer. The ABEL trap operates by tracking the Brownian motion of a single fluorescent particle in solution, and applying a time-dependent electric field designed to induce an electrokinetic drift that cancels the Brownian motion. The trapping strength of the ABEL trap is limited by the latency of the feedback loop. In previous versions of the trap, this latency was set by the finite frame rate of the camera used for video-tracking. In the present system, the motion of the particle is tracked entirely in hardware (without a camera or image-processing software) using a rapidly rotating laser focus and lock-in detection. The feedback latency is set by the finite rate of arrival of photons. We demonstrate trapping of individual molecules of the protein GroEL in buffer, and we show confinement of single fluorophores of the dye Cy3 in water.

© 2008 Optical Society of America

OCIS Codes
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: January 3, 2008
Revised Manuscript: April 23, 2008
Manuscript Accepted: April 30, 2008
Published: May 1, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Adam E. Cohen and W. E. Moerner, "Controlling Brownian motion of single protein molecules and single fluorophores in aqueous buffer," Opt. Express 16, 6941-6956 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-10-6941


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