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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 6 — Jun. 17, 2008

Fluorescence endomicroscopy with structured illumination

Nenad Bozinovic, Cathie Ventalon, Tim Ford, and Jerome Mertz  »View Author Affiliations


Optics Express, Vol. 16, Issue 11, pp. 8016-8025 (2008)
http://dx.doi.org/10.1364/OE.16.008016


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Abstract

We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (~2.6µm) optically sectioned fluorescence images over a field of view of about 240µm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.

© 2008 Optical Society of America

OCIS Codes
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 7, 2008
Revised Manuscript: April 17, 2008
Manuscript Accepted: April 21, 2008
Published: May 19, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Nenad Bozinovic, Cathie Ventalon, Tim Ford, and Jerome Mertz, "Fluorescence endomicroscopy with structured illumination," Opt. Express 16, 8016-8025 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-11-8016


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