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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics


  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 8 — Aug. 18, 2008

A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view.

Y. Kremer, J.-F. Léger, R. Lapole, N. Honnorat, Y. Candela, S. Dieudonné, and L. Bourdieu  »View Author Affiliations

Optics Express, Vol. 16, Issue 14, pp. 10066-10076 (2008)

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Acousto-optic deflectors (AOD) are promising ultrafast scanners for non-linear microscopy. Their use has been limited until now by their small scanning range and by the spatial and temporal dispersions of the laser beam going through the deflectors. We show that the use of AOD of large aperture (13mm) compared to standard deflectors allows accessing much larger field of view while minimizing spatio-temporal distortions. An acousto-optic modulator (AOM) placed at distance of the AOD is used to compensate spatial and temporal dispersions. Fine tuning of the AOM-AOD setup using a frequency-resolved optical gating (GRENOUILLE) allows elimination of pulse front tilt whereas spatial chirp is minimized thanks to the large aperture AOD.

© 2008 Optical Society of America

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(230.1040) Optical devices : Acousto-optical devices
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: March 20, 2008
Revised Manuscript: June 2, 2008
Manuscript Accepted: June 2, 2008
Published: June 23, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Y. Kremer, J.-F. Léger, R. Lapole, N. Honnorat, Y. Candela, S. Dieudonné, and L. Bourdieu, "A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view," Opt. Express 16, 10066-10076 (2008)

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