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Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 8 — Aug. 18, 2008

Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

David Liao, Peter Galajda, Robert Riehn, Rob Ilic, Jason L. Puchalla, Howard G. Yu, Harold G. Craighead, and Robert H. Austin  »View Author Affiliations


Optics Express, Vol. 16, Issue 14, pp. 10077-10090 (2008)
http://dx.doi.org/10.1364/OE.16.010077


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Abstract

Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ~17 mm/s. We derive an analytic scaling relationship δ k ON k ON = δ k OFF k OFF ~ k ON + k OFF k ON 0.1 D B D F D B e SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

© 2008 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(230.7370) Optical devices : Waveguides
(300.2530) Spectroscopy : Fluorescence, laser-induced
(220.4241) Optical design and fabrication : Nanostructure fabrication

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 31, 2008
Revised Manuscript: May 14, 2008
Manuscript Accepted: June 19, 2008
Published: June 23, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
David Liao, Peter Galajda, Robert Riehn, Rob Ilic, Jason L. Puchalla, Howard G. Yu, Harold G. Craighead, and Robert H. Austin, "Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides," Opt. Express 16, 10077-10090 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-14-10077


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