Miniaturized multiphoton microscope with a 24Hz frame-rate
Optics Express, Vol. 16, Issue 14, pp. 10501-10506 (2008)
http://dx.doi.org/10.1364/OE.16.010501
Enhanced HTML
Acrobat PDF (329 KB)
Abstract
With miniaturized tube lenses and a micro-electro-mechanical system (MEMS) mirror, we constructed a miniaturized multiphoton microscope system. Through a two-dimensional asynchronous scanning of the MEMS mirror, 24Hz frame rate can be realized. With a high numerical aperture objective, sub-micron resolution can also be achieved at the same time.
© 2008 Optical Society of America
OCIS Codes
(190.3970) Nonlinear optics : Microparticle nonlinear optics
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Microscopy
History
Original Manuscript: January 15, 2008
Revised Manuscript: May 19, 2008
Manuscript Accepted: May 21, 2008
Published: June 30, 2008
Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Tzu-Ming Liu, Ming-Che Chan, I-Hsiu Chen, Shih-Hsuan Chia, and Chi-Kuang Sun, "Miniaturized multiphoton microscope with a 24Hz frame-rate," Opt. Express 16, 10501-10506 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-14-10501
Sort: Year | Journal | Reset
References
- L. Fu, A. Jain, H. Xie, C. Cranfield, and M. Gu, "Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror," Opt. Express 14, 1027-1032 (2006). [CrossRef] [PubMed]
- W. Piyawattanametha, R. P. J. Barretto, T. H. Ko, B. A. Flusberg, E. D. Cocker, H. Ra, D. Lee, O. Solgaard, and M. J. Schnitzer, "Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror," Opt. Lett. 31, 2018-2020 (2006). [CrossRef] [PubMed]
- H. J. Shin, M. C. Pierce, D. Lee, H. Ra, O. Solgaard, and R. Richards-Kortum, "Fiber-optic confocal microscope using a MEMS scanner and miniature objectives lens," Opt. Express 15, 9113-9122 (2007). [CrossRef] [PubMed]
- B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, "In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microscope, " Opt. Lett. 30, 2272-2274 (2005). [CrossRef] [PubMed]
- W. Göbel, J. N. D. Kerr, A. Nimmerjahn, and F. Helmchen, " Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective," Opt. Lett. 29, 2521-2523 (2004). [CrossRef] [PubMed]
- J. C. Jung and M. J. Schnitzer,"Multiphoton endoscopy," Opt. Lett. 28, 9902-904 (2003).
- D. Bird and M. Gu, "Two-photon fluorescence endoscopy with a micro-optic scanning head," Opt. Lett. 28, 1552-1554 (2003). [CrossRef] [PubMed]
- M. J. Levene, D. A. Dombeck, K. A. Kasischke, R. P. Molly, and W. W. Webb, "In vivo multiphoton microscopy of deep brain tissue," J. Neurophysiol. 91, 1908-1912 (2003). [CrossRef] [PubMed]
- R. M. Williams, W. R. Ziptel, and W. W. Webb, "Interpretating second-harmonic generation images of collagen I fibrils," Biophys. J. 88, 1377-1386 (2005). [CrossRef]
- T. A. Theodossiou, C. Thrasivoulou, C. Ekwobi, and D. L. Becker, "Second harmonic generation confocal microscopy of collagen type I from rat tendon cryosections," Biophys. J. 91, 4665-4677 (2006). [CrossRef] [PubMed]
- L. Fu and M. Gu, " Polarization anisotropy in fiber-optic second harmonic generation microscopy," Opt. Express 16, 5000-5006 (2008). [CrossRef] [PubMed]
Cited By |
OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.
Multimedia
| Multimedia Files | Recommended Software |
| » Media 1: AVI (2203 KB) |





OSA is a member of 