OSA's Digital Library

Virtual Journal for Biomedical Optics

Virtual Journal for Biomedical Optics

| EXPLORING THE INTERFACE OF LIGHT AND BIOMEDICINE

  • Editor: Gregory W. Faris
  • Vol. 3, Iss. 8 — Aug. 18, 2008

Miniaturized multiphoton microscope with a 24Hz frame-rate

Tzu-Ming Liu, Ming-Che Chan, I-Hsiu Chen, Shih-Hsuan Chia, and Chi-Kuang Sun  »View Author Affiliations


Optics Express, Vol. 16, Issue 14, pp. 10501-10506 (2008)
http://dx.doi.org/10.1364/OE.16.010501


View Full Text Article

Enhanced HTML    Acrobat PDF (329 KB) Open Access





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

With miniaturized tube lenses and a micro-electro-mechanical system (MEMS) mirror, we constructed a miniaturized multiphoton microscope system. Through a two-dimensional asynchronous scanning of the MEMS mirror, 24Hz frame rate can be realized. With a high numerical aperture objective, sub-micron resolution can also be achieved at the same time.

© 2008 Optical Society of America

OCIS Codes
(190.3970) Nonlinear optics : Microparticle nonlinear optics
(190.4710) Nonlinear optics : Optical nonlinearities in organic materials
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 15, 2008
Revised Manuscript: May 19, 2008
Manuscript Accepted: May 21, 2008
Published: June 30, 2008

Virtual Issues
Vol. 3, Iss. 8 Virtual Journal for Biomedical Optics

Citation
Tzu-Ming Liu, Ming-Che Chan, I-Hsiu Chen, Shih-Hsuan Chia, and Chi-Kuang Sun, "Miniaturized multiphoton microscope with a 24Hz frame-rate," Opt. Express 16, 10501-10506 (2008)
http://www.opticsinfobase.org/vjbo/abstract.cfm?URI=oe-16-14-10501


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. L. Fu, A. Jain, H. Xie, C. Cranfield, and M. Gu, "Nonlinear optical endoscopy based on a double-clad photonic crystal fiber and a MEMS mirror," Opt. Express 14, 1027-1032 (2006). [CrossRef] [PubMed]
  2. W. Piyawattanametha, R. P. J. Barretto, T. H. Ko, B. A. Flusberg, E. D. Cocker, H. Ra, D. Lee, O. Solgaard, and M. J. Schnitzer, "Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two-dimensional scanning mirror," Opt. Lett. 31, 2018-2020 (2006). [CrossRef] [PubMed]
  3. H. J. Shin, M. C. Pierce, D. Lee, H. Ra, O. Solgaard, and R. Richards-Kortum, "Fiber-optic confocal microscope using a MEMS scanner and miniature objectives lens," Opt. Express 15, 9113-9122 (2007). [CrossRef] [PubMed]
  4. B. A. Flusberg, J. C. Jung, E. D. Cocker, E. P. Anderson, and M. J. Schnitzer, "In vivo brain imaging using a portable 3.9 gram two-photon fluorescence microscope, " Opt. Lett. 30, 2272-2274 (2005). [CrossRef] [PubMed]
  5. W. Göbel, J. N. D. Kerr, A. Nimmerjahn, and F. Helmchen, " Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective," Opt. Lett. 29, 2521-2523 (2004). [CrossRef] [PubMed]
  6. J. C. Jung and M. J. Schnitzer,"Multiphoton endoscopy," Opt. Lett. 28, 9902-904 (2003).
  7. D. Bird and M. Gu, "Two-photon fluorescence endoscopy with a micro-optic scanning head," Opt. Lett. 28, 1552-1554 (2003). [CrossRef] [PubMed]
  8. M. J. Levene, D. A. Dombeck, K. A. Kasischke, R. P. Molly, and W. W. Webb, "In vivo multiphoton microscopy of deep brain tissue," J. Neurophysiol. 91, 1908-1912 (2003). [CrossRef] [PubMed]
  9. R. M. Williams, W. R. Ziptel, and W. W. Webb, "Interpretating second-harmonic generation images of collagen I fibrils," Biophys. J. 88, 1377-1386 (2005). [CrossRef]
  10. T. A. Theodossiou, C. Thrasivoulou, C. Ekwobi, and D. L. Becker, "Second harmonic generation confocal microscopy of collagen type I from rat tendon cryosections," Biophys. J. 91, 4665-4677 (2006). [CrossRef] [PubMed]
  11. L. Fu and M. Gu, " Polarization anisotropy in fiber-optic second harmonic generation microscopy," Opt. Express 16, 5000-5006 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Supplementary Material


» Media 1: AVI (2203 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited